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Titlebook: In Living Color; Protocols in Flow Cy Rochelle A. Diamond (Member of the Professional St Book 2000 Springer-Verlag Berlin Heidelberg 2000 A

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Detection of Cell Surface Antigens Using Antibody-conjugated Fluorospheres and Flow Cytometry from those of conventional fluorochromes expanding the multicolour capabilities. This protocol illustrates the use of antibody-conjugated fluorospheres (ACF) for detection of cell surface antigens that may be used in combination with multicolour applications using standard flow cytometers.
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How Flow Cytometers Work — and Don’t Worku won’t get many of the details from these few pages, but I’ll try to point you in the right direction; I have written about the subject at much greater length, if you’re interested in pursuing things further.
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Quality Control Guidelines for Research Flow Cytometryelevant parameters within a sample, between samples, between laboratories, over time. For an in depth review of these issues see reference one. which provides a good bibliography on quality control for clinical applications.
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The Basics of Staining for Cell Surface Proteinssually required to make biochemical variation “visible” to the photodetectors of a flow cytometer. By making use of fluorescent stains that are specific for different molecules, we can use a flow cytometer to assay a large array of different cell constituents.
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