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Titlebook: In Living Color; Protocols in Flow Cy Rochelle A. Diamond (Member of the Professional St Book 2000 Springer-Verlag Berlin Heidelberg 2000 A

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发表于 2025-3-21 16:36:54 | 显示全部楼层 |阅读模式
书目名称In Living Color
副标题Protocols in Flow Cy
编辑Rochelle A. Diamond (Member of the Professional St
视频video
概述Flow cytometry and cell sorting are becoming increasingly important in biological research.Step-by-step approach to the methodology for measuring various cellular attributes.Includes supplementary mat
丛书名称Springer Lab Manuals
图书封面Titlebook: In Living Color; Protocols in Flow Cy Rochelle A. Diamond (Member of the Professional St Book 2000 Springer-Verlag Berlin Heidelberg 2000 A
描述Advances in the field of cell biology have always been closely related to the development of quantitative analytical methods that can be applied to individual cells or cell organelles. Almost from the early stages following the invention of the microscope, the investigator has been keenly interested in obtaining informa­ tion on the functionality of single cells and how cells perform under different sets of experimental conditions. Although cells could be viewed in the microscope for a few hundred years, only since the relatively recent application of autoradiography did we come to realize that, although cells may visually appear very much alike, they are quite different in their functional capacity. The quest to understand these differences in a cell population lead to a new series of techniques for labeling and quantitating DNA content and similar approaches have driven the develop­ ment of methods for analyzing various other cellular properties. The development of new analytical techniques follows the age old pattern of applying successes of the past with current inno­ vation, logic and new biological information. Results from auto­ radiography expanded the concept of the cell c
出版日期Book 2000
关键词Antigen; Calcium; DNA; Endoplasmatisches Reticulum; RNA; Termination; core facility; fluoresence activated
版次1
doihttps://doi.org/10.1007/978-3-642-57049-0
isbn_softcover978-3-642-62978-5
isbn_ebook978-3-642-57049-0
copyrightSpringer-Verlag Berlin Heidelberg 2000
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发表于 2025-3-21 23:41:27 | 显示全部楼层
The Colorful History of Flow Cytometry flow cytometry, many of them listed in the references of this manual. You can read them as you wish, at your leisure. However, it is a good idea to look at the varied and colorful past of this technology and how it has developed into the tool we use today. A quick look at the historical influences
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How Flow Cytometers Work — and Don’t Workf the instrument, you may not care much about how flow cytometers work. A lot of highly competent molecular biologists, immunologists, etc., look at the flow cytometer as a tool, and just want to see samples go in and pretty pictures and numbers come out. This works remarkably well if you’re doing t
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Flow Cytometry Standard (FCS) Data File Format A data files standard is essentially a list of rules describing how the computer data file should be organized. When adhered to, the rules yield a defined and uniform computer file structure. The letters ‘FCS’ refer specifically to the Flow Cytometry Standard format, which is a comprehensive data-f
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Guidelines to Improve Flow Cytometry Data Display and Interpretationcal programs such as cancer, gene therapy and transplantation. While investigators in these fields seek to use the advantages of FCM to further their work, without good background information about FCM, the experiments conducted may be poorly controlled or be misinterpreted. In addition, this FCM da
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Quality Control Guidelines for Research Flow Cytometrynts and reagents. In clinical laboratories, quality control methods require rigorous documentation, certification, and monitoring for reproducibility and accuracy. This entails validation of chain of custody information (sample source, collection procedures, transportation and storage of viable samp
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Sample Preparation and Cell Surface Stainingd et al present a discussion on various methodologies to make the all-important single cell mono-dispersed samples. They provide protocols for cleaning up debris, getting rid of dead cells, and ways to enrich for the investigator’s particular population of interest. Alice Givans follows with an in-d
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