Titlebook: Immunoproteomics; Methods and Protocol Kelly M. Fulton,Susan M. Twine Book 2019Latest edition Springer Science+Business Media, LLC, part of

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书目名称Immunoproteomics影响因子(影响力)




书目名称Immunoproteomics影响因子(影响力)学科排名




书目名称Immunoproteomics网络公开度




书目名称Immunoproteomics网络公开度学科排名




书目名称Immunoproteomics被引频次




书目名称Immunoproteomics被引频次学科排名




书目名称Immunoproteomics年度引用




书目名称Immunoproteomics年度引用学科排名




书目名称Immunoproteomics读者反馈




书目名称Immunoproteomics读者反馈学科排名

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Immunoproteomics Methods and Techniques,s or cancerous cells. The study of immune biomarkers or antigens is not new, and classical methods such as agglutination, enzyme-linked immunosorbent assay, or Western blotting have been used for many years to study the immune response to vaccination or disease. However, in many of these traditional

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Classical Immunoproteomics: Serological Proteome Analysis (SERPA) for Antigen Identification,protective antigens, vaccine development, and discovery of biomarkers for early diagnosis. Proteomic approaches, including serological proteome analysis (SERPA), have been used to identify the repertoire of immunoreactive proteins in various diseases. In this chapter, we provide an outline of the SE

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,Enriching for Low-Abundance Serum Proteins Using ProteoMiner™ and Protein-Level HPLC,sult of such an immune response can shed light on the mechanism of disease or serve as biomarkers. These biomarkers can be used in a diagnostic capacity or as correlates of protection following vaccination. Protein levels in the circulatory system are considered representative of the system as a who

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,Identification of the Antigen Content of Electroimmunoprecipitates,main specificity in reactions that are best visualized by gel electroimmunoprecipitation methods (e.g., when analyzing complex antigen mixtures). The great advantage of gel immunoprecipitation approaches is that each immunoprecipitate contains antigen in a pure form and that the precipitate is separ

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Detection of Human Norovirus-Specific Antibodies Using the Luciferase Immunoprecipitation System (Luminescence emitted by the reporter enzyme . luciferase (Ruc) fused to an antigen of interest. The LIPS assay can be utilized as a high-throughput and sensitive serological method for profiling serum antibodies recognizing diverse antigens. This chapter provides a detailed protocol for detecting hum

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Intact Mass Spectrometry Analysis of Immuno-Isolated Human Therapeutic Antibodies from Serum, and downstream processing technologies. The structural diversity of therapeutic antibodies has also evolved with the development of bispecific (and multispecific) antibodies and antibody-drug conjugates. With increased structural complexities and multi-modularity, there is a need to demonstrate tha

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Antigen Identification for Cell-Binding Antibodies Using Ligand-Directed Crosslinking and Biotin Tr we describe a protocol that uses ligand-directed crosslinking with the aminooxy-sulfhydryl-biotin (ASB) trifunctional crosslinker followed by a proteomic analysis to identify the cellular receptors for orphan ligands. We describe the synthesis of the ASB crosslinker, labelling of the ligand with AS