Titlebook: Immunoelectron Microscopy; Methods and Protocol Steven D. Schwartzbach,Tetsuaki Osafune Book 2010 Springer Science+Business Media, LLC 2010

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Thomas Schikorskirivation and application of nucleation theorems that can be used to extract microscopic cluster properties from nucleation rate measurements. ..The assumptions and appr978-3-642-06721-1978-3-540-31218-5

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Haruo Hagiwara,Takeo Aoki,Takeshi Suzuki,Kuniaki Takatarivation and application of nucleation theorems that can be used to extract microscopic cluster properties from nucleation rate measurements. ..The assumptions and appr978-3-642-06721-1978-3-540-31218-5

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Book 2010ntigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Mole

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Transient Expression of Epitope-Tagged Proteins in Mammalian Cellsor to immunoelectron microscopy, expression and localization of tagged proteins can be assessed by Western blotting and immunofluorescence. Furthermore, specialized fluorescent tags, such as the green fluorescent protein (GFP), can be used to rapidly screen for transfection efficiency and localizati

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Pre-embedding Immunogold Localization of Antigens in Mammalian Brain Slicesructure. The procedure employs thick sectioning using a vibratome, permeabilization of membranes with Triton X-100, and immunolabeling with fluorescently conjugated Nanogold antibodies, followed by gold enhancement and embedding for electron microscopy. We also discuss some limitations inherent to p

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In Vivo Cryotechniques for Preparation of Animal Tissues for Immunoelectron Microscopyivo cryotechnique,” and every components of the cells and tissues are maintained in situ at the time of freezing. Thus, ischemic or anoxic effects are minimized on immunohistochemical localization of the components. Another new “cryobiopsy” technique will be useful for capturing time-dependent morph