| 书目名称 | Immunoelectron Microscopy |
| 副标题 | Methods and Protocol |
| 编辑 | Steven D. Schwartzbach,Tetsuaki Osafune |
| 视频video | http://file.papertrans.cn/463/462137/462137.mp4 |
| 概述 | Presents a wide range of Cryo and chemical fixation methods for single cells, plant, and animal tissue.Contains Immunogold labeling methods for transmission and scanning electron microscopy.Includes c |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells,methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Mole |
| 出版日期 | Book 2010 |
| 关键词 | Antigen; Fixation protocols; Macromolecular functions; Monoclonal Antibodies; Organelle; Pre- and post-em |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-1-60761-783-9 |
| isbn_softcover | 978-1-4939-5758-3 |
| isbn_ebook | 978-1-60761-783-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Springer Science+Business Media, LLC 2010 |
|Archiver|手机版|小黑屋|
派博传思国际
( 京公网安备110108008328)
GMT+8, 2025-8-19 03:48
发表于 2025-3-21 18:44:34
