Titlebook: Immunocytochemical Methods and Protocols; Lorette C. Javois Book 19951st edition Humana Press 1995

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Conjugation of Fluorochromes to Antibodies,ed. The primary antigenic site is thus revealed. A major advantage of the indirect method is the enhanced sensitivity. In addition, a labeled secondary antibody can be used to locate any number of primary antibodies raised in the same animal species without the necessity of labeling each primary antibody.

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Overview of Cell Fixation and Permeabilization,d proteins and carbohydrates. This chapter will review some of the advantages and drawbacks of common chemical fixation and permeabilization methods used for immunolocalization at the level of light microscopy.

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Book 19951st editionring this time the use of immunocytochemical meth­ ods has spread to a wide array of biological disciplines. Early applica­ tions focused on the detection of microbial antigens in tissues, while more recent applications have used monoclonal antibodies to study cell differentiation during embryonic d

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