Titlebook: Immunocytochemical Methods and Protocols; Lorette C. Javois Book 19951st edition Humana Press 1995

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书目名称Immunocytochemical Methods and Protocols影响因子(影响力)




书目名称Immunocytochemical Methods and Protocols影响因子(影响力)学科排名




书目名称Immunocytochemical Methods and Protocols网络公开度




书目名称Immunocytochemical Methods and Protocols网络公开度学科排名




书目名称Immunocytochemical Methods and Protocols被引频次




书目名称Immunocytochemical Methods and Protocols被引频次学科排名




书目名称Immunocytochemical Methods and Protocols年度引用




书目名称Immunocytochemical Methods and Protocols年度引用学科排名




书目名称Immunocytochemical Methods and Protocols读者反馈




书目名称Immunocytochemical Methods and Protocols读者反馈学科排名

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Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration,um sulfate fractionation, is generally employed as the initial step in the isolation of crude antibodies from serum or ascitic fluid (.#x2013;.). Ammonium sulfate precipitation, in many instances, is still the method of choice because it offers a number of advantages. Ammonium sulfate fractionation

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Thyroiditis

Purification of Antibodies Using Affinity Chromatography,is type of separation, unlike other chromatographic methods, uses the intrinsic biological activity of a molecule to bind to a substrate, hapten, or antigen. Principles of matrix selection, gel preparation, and coupling of ligands have been reviewed extensively by Ostrove (.). Antibody affinity chro

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albuminuria

Conjugation of Fluorochromes to Antibodies,t first, the specific antibody itself was labeled and applied to the tissue section to identify the antigenic sites (direct method) (.). Later, the more sensitive and versatile indirect method (.) was introduced. The primary, unlabeled, antibody is applied to the tissue section, and the excess is wa

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Biotinylation of Antibodies,ty chromatography, and many other areas (.) (.. and .). It was first exploited in immunocytochemical applications in the mid-1970s (.,.), and has since been commonly used to localize antigens in cells and tissues. In this technique, a biotinylated primary or secondary antibody is first applied to th

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Processing of Cell-Touch Preparations,ing into them precludes the use of frozen sections. Touch preparations or imprints enable the examination of the whole cell apart from the tissue aspect. The cells are obtained by touching a wet tissue with a glass slide. Cells adhere to the glass in roughly the same orientation as they exist on the