Titlebook: Immuno-Oncology; Cellular and Transla Seng-Lai Tan Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 Cancer immu

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Validation of an Image-Based 3D Natural Killer Cell-Mediated Cytotoxicity Assay,chnology induced HCT116 cells to aggregate into tumoroids over time, which became the target cells during the cytotoxicity assay. Cytotoxicity was assessed by both phosphatidyl serine exposure (apoptosis) and plasma membrane rupture (necrosis) using fully automated workflows. Cytotoxicity was quanti

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Considerations in Developing Reporter Gene Bioassays for Biologics,ial in biologic drug development. Here we describe a simple bioluminescent reporter gene bioassay for assessing biologics targeting immune checkpoints without the complexity and variability of more traditional assay systems. This chapter provides an overview of key considerations in reporter gene bi

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Miniaturized Single Cell Imaging for Developing Immuno-Oncology Combinational Therapies,onventional chemotherapies and to single agents, remains a major hurdle. A current approach to combating drug resistance and risk of cancer recurrence is to find combinations of drugs that effectively target multiple functional pathways, increasing the prospect of providing more durable cancer cell

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Precision Medicine: The Function of Receptor Occupancy in Drug Development,le to simultaneously measure over 50 parameters at the single-cell level and within complex mixtures of cells with significant precision. Along with immunophenotyping and functional testing used for clinical immune monitoring, receptor occupancy (RO) assays have become a standard application in flow

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In Vitro Assays for Assessing Potential Adverse Effects of Cancer Immunotherapeutics,to activate the immune system and elicit anticancer immune responses, a majority of adverse effects noted with CIT drugs are related to the molecule’s pharmacology and exaggeration of pharmacological responses. A major challenge for CIT is that nonclinical toxicity studies utilizing healthy animals

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The Quest for the Next-Generation of Tumor Targets: Discovery and Prioritization in the Genomics Ermajority of cancer patients, especially those with solid tumors. Chimeric antigen receptor (CAR) T-cell therapies, for example, are far from being successfully implemented..A bottleneck in modern immunotherapy of cancer is the identification of molecules that allow effective targeting of the tumor w

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1557-2153 developing next generation translational assaysThis book serves as a guide for identifying and applying commonly used cell-based translational assays as well as for assessing the therapeutic potential of new immuno-oncology therapeutics and advancing their mechanism of action. The detailed chapters