Titlebook: Immune Receptors; Methods and Protocol Jonathan Rast,Katherine Buckley Book 2022Latest edition Springer Science+Business Media, LLC, part o

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An Imaging Flow Cytometry Protocol for Studying Immunoregulatory Receptor-Mediated Regulation of Phimaging capabilities of microscopy with rapid, high-throughput data acquisition attributes of standard flow cytometers. Here, we describe a detailed method for co-expressing stimulatory and inhibitory immunoregulatory receptor-types in AD293 cells and then measuring receptor cross-talk during the re

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Single-Cell Sequencing for Everybody,n from neurobiology to developmental biology from immunology to tumor biology have been approaching this technique. For someone new to this field that wants to investigate heterogeneity in what appears to be a single-cell population, the choice of the best protocol can be difficult, due to the high

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Fetal Thymic Organ Culture (FTOC) Optimized for Gamma-Delta T Cell Studies,bles studies of genetically altered mice that are embryonic or neonatal lethal, in addition to bypassing the complication of migration of successive waves of T cells out of the thymus. The hanging drop method involves depletion of thymocytes from host lobes using deoxyguanosine, followed by reconsti

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1064-3745 troubleshooting and avoiding known pitfalls..Cutting-edge and authoritative, .Immune Receptors: Methods and Protocols, Second Edition. is a valuable resource for scientists and researchers interested in learning more about this developing field.  .978-1-0716-1946-9978-1-0716-1944-5Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Christine Arnold,Kornelia Ellwanger,Thomas A. Kuferlarly boundaries, but also seeks to escape deeply entrenched notions of citizenship as either a form of political participation or legal status... .978-3-031-48563-3978-3-031-48561-9Series ISSN 2945-5936 Series E-ISSN 2945-5944

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Micropatterned Ligand Arrays to Investigate Spatial Regulation of Cellular Signaling Initiated by C dimensions of the signaling complexes and their dispersal across the cell surface. To overcome these limitations in our model system, we use microfabricated surfaces containing spatially defined ligands to cluster and activate IgE receptors (FcεRI), which initiate allergic responses by mast cells.

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Identification of Glycan-Specific Variable Lymphocyte Receptors Using Yeast Surface Display and Glyymphocyte receptor B, or VLRBs. The anti-carbohydrate VLRBs are isolated using a yeast surface display (YSD) expression platform and enriched by binding to glycan microarrays through the anti-glycan VLRB. This enables both the initial identification and enrichment of individual yeast clones against