Titlebook: Immune Receptors; Methods and Protocol Jonathan Rast,Katherine Buckley Book 2022Latest edition Springer Science+Business Media, LLC, part o

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Generation of VNAR Libraries from Immunized Sharks and Selection of Target-Specific Clones,anging immunoglobulins (Igs or antibodies) have also been found. Alongside their conventional (heavy-light chain) isotypes, IgM and IgW, sharks produce the novel isotype, IgNAR, a heavy-chain homodimer. Naturally lacking light chains, antigen binding is mediated by two highly soluble and independent

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Cell Aggregation Assays for Homophilic Interactions Between Cell Surface Proteins,teractions can occur between different but complementary proteins such as a ligand and receptor, or between the same protein acting as both ligand and receptor. The cell aggregation assay is a straightforward technique to identify homophilic interactions from such proteins. Here we describe the proc

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A Role for Secreted Immune Effectors in Microbial Biofilm Formation Revealed by Simple In Vitro Assenvironments by exuding exopolysaccharides and other macromolecules such as proteins, lipids, and even DNA. It is not just bacteria, but fungi such as yeast, that form these adherent interacting communities. Historically, biofilms have been studied in the context of pathogenesis, but only recently i

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Production and Characterization of SIV-Specific CAR/CXCR5 T Cells,s, NK cells, or peripheral blood mononuclear cells (PBMC) may be modified to express HIV-specific CARs as well as follicular homing molecules such as CXCR5 to target the virally infected T follicular helper cells that concentrate within B cell follicles during HIV infection. This chapter outlines me

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Engineering IgG1 Fc Domains That Activate the Complement System,ation of the classical complement pathway and through the binding to Fcγ receptors (FcγRs) which mediate clearance of targeted cells by antibody-dependent cellular cytotoxicity (ADCC) and antibody-dependent cellular phagocytosis (ADCP) by effector cells such as macrophages, NK cells, and other leuko