Titlebook: Immune Checkpoint Blockade; Methods and Protocol Yago Pico de Coaña Book 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Expansion of Tumor-Infiltrating Lymphocytes from Melanoma Tumorsctors. This second expansion step is usually around 2 weeks in length. Here will be described a REP that uses soluble anti-CD3 antibodies for TCR triggering, irradiated peripheral blood mononuclear cells (PBMC) as feeder cells, and IL-2 as the T cell growth factor. Furthermore, the described protoco

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Expansion and Determination of Antigen-Reactive T Cells by Flow Cytometryo be characterized. Furthermore, this method simultaneously detects CD4+ and CD8+ T cells that produce cytokines upon encounter with antigen and thereby provides a functional insight into the behavior of the responding T cells. In our case, we have investigated the pro- or anti-inflammatory cytokine

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Assessing Canonical and Adaptive Natural Killer Cell Function in Suppression Assays In Vitro further be utilized to study the effects of immune checkpoint inhibitors in vitro and ex vivo. We focus on the differences between canonical NK cells and the newly identified subset of NK cells termed “adaptive NK cells.” These cells are induced by cytomegalovirus (CMV) in CMV-seropositive individu

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Shi Yong Neo,Aine O’Reilly,Yago Pico de Coañagies, alongside new policies for smarter, proactive citizens – making them a valuable resource for researchers and policymakers alike..978-3-319-87052-6978-3-319-61322-2Series ISSN 2190-3018 Series E-ISSN 2190-3026

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Alexander Martens,Graham Pawelec,Christopher Shipp

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GRE