Titlebook: Imaging Mass Spectrometry; Methods and Protocol Laura M Cole,Malcolm R Clench Book 2023Latest edition The Editor(s) (if applicable) and The

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Cytological Cytospin Preparation for the Spatial Proteomics Analysis of Thyroid Nodules Using MALDI In particular, spatial proteomics using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) represents one of the most promising avenues, owing to its capability to map the distribution of hundreds of proteins within a complex cytological background in a multiplexed

Cytology

Matrix Effects Free Imaging of Thin Tissue Sections Using Pneumatically Assisted Nano-DESI MSI,nization of the continuously altered and complex chemistry in each pixel can introduce artifacts that result in skewed molecular distributions in the compiled ion images. These artifacts are known as matrix effects. Mass spectrometry imaging using nanospray desorption electrospray ionization (nano-D

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Sample Preparation for Metabolite Detection in Mass Spectrometry Imaging,ogical functions and disease development. When studying heterogeneous tissue samples, mass spectrometry imaging (MSI) is a valuable tool as it conserves the spatial distribution of analytes on tissue sections. A large proportion of metabolites are, however, small and polar, making them vulnerable to

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Multimodal Mass Spectrometry Imaging of an Aggregated 3D Cell Culture Model,ection of compounds including metabolites, lipids, proteins, and metal isotopes allows for a more holistic understanding of tissue microenvironments. Universal sample preparation is a primary enabler for samples of the same set to be run across multiple modalities. Using the same method and material

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