Titlebook: Hydrocarbon and Lipid Microbiology Protocols; Genetic, Genomic and Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale Book 2017 Springer-Ve

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Preparation and Analysis of Metatranscriptomic Libraries in Petroleum Hydrocarbon Microbe Systems,tential catalogue of interacting elements that can be used to define metabolic relationships between microbes. These metatranscriptomic analyses can also be coupled with metagenomic sequencing and statistical models to provide an in-depth approach to assemblage systems biology. In this work, we deta

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Protein Extraction from Contaminated Soils and Sediments,ral methods for efficient extraction of proteins from contaminated soils or sediments. Such methods allow obtaining high protein yields which could be used for shotgun proteomic approaches or any kind of proteomic approach, including enzymatic activity tests. These approaches include those based on

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Analysis of the Regulation of the Rate of Hydrocarbon and Nutrient Flow Through Microbial Communitinumbers of a specific species or functional group and the flux through those cells, under two or more different conditions, enables determining the regulation coefficients. The slope in a double logarithmic plot of the cell numbers against the corresponding flux through them provides the population-

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Constructing and Analyzing Metabolic Flux Models of Microbial Communities,ch other and with the gut environment, exploring the trade-offs in information provided by applying each metabolic flux modeling approach. Overall, we conclude that no single approach is better than the others, and often there is much to be gained by applying multiple approaches synergistically when

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Book 2017es and of the types of microbe involved in them. Protocols for the analysis of nutrient flow through microbial communities and for the modelling of dynamic physiological interactions in communities are also provided. Lastly, the book presents a protocol for the quantitative assessment of permissiven