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Titlebook: Hydrocarbon and Lipid Microbiology Protocols; Genetic, Genomic and Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale Book 2017 Springer-Ve

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Prokaryotic Metatranscriptomics,nologies and in software for analysis have made metatranscriptomics a very amenable technique, soon to be routinely used. In this chapter, we illustrate the steps needed to perform a metatranscriptomic experiment, from the collection of the samples to the production of the final results. The full pr
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Defining a Pipeline for Metaproteomic Analyses, meta aspect of this omic approach is still in its infancy and subject to methodological and data analysis improvements over the next years. The need to define correct methods for such analyses is essential before hypothesis-driven projects can be addressed. Here we discuss the current state of meta
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Metabolic Profiling and Metabolomic Procedures for Investigating the Biodegradation of Hydrocarbonstabolic fate of hydrocarbons in systems that range in complexity from individual bacterial cells to natural or man-made environmental compartments. Such studies can be divided into targeted analysis, traditionally performed using gas chromatography/mass spectrometry, and untargeted analysis, for whi
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Protocol for Performing Protein Stable Isotope Probing (Protein-SIP) Experiments,ncept is based on the application of stable isotope-labeled compounds, .C or .N as substrates supplied to microbial communities grown within microcosm systems. Microorganisms capable of utilizing these substrates become labeled upon metabolism and biosynthesis. The sum of all proteins represents the
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Protein Extraction from Contaminated Soils and Sediments,s and marine sediments. They may primarily be considered as an ecological problem, but at the same time constituted a factor influencing microbial populations and the biodegradation processes they mediate. Several strategies have been used to study the relationship between the PAHs input and ecosyst
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