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Titlebook: Hydrocarbon and Lipid Microbiology Protocols; Synthetic and System Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale Book 2016 Springer-Ve

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发表于 2025-3-21 19:33:55 | 显示全部楼层 |阅读模式
书目名称Hydrocarbon and Lipid Microbiology Protocols
副标题Synthetic and System
编辑Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale
视频video
概述Offers readily-reproducible step-by-step laboratory methods.Provides helpful tips and tricks supporting the protocols.Includes troubleshooting advice.Contains practical laboratory guidance to promote
丛书名称Springer Protocols Handbooks
图书封面Titlebook: Hydrocarbon and Lipid Microbiology Protocols; Synthetic and System Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale Book 2016 Springer-Ve
描述This Volume presents protocols for systems and synthetic biology applications in the field of hydrocarbon and lipid microbiology. It complements another Volume that describes generic protocols for wet experimental and computer-based systems and synthetic biology studies. The protocols in this Volume demonstrate how to employ systems and synthetic biology approaches in the design of microbes for the production of esters, isoprenoids, hydrophobic polymers, rhamnolipid biosurfactant, and peptide antimicrobial and thioether-stabilised molecules. Also presented is a protocol for the engineering of transcription factor-based biosensors for intracellular products, and another for the creation of a synthetic hydroxylase with novel activity for the selective oxyfunctionalisation of linear alkanes..Hydrocarbon and Lipid Microbiology Protocols.There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivativesand lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environment
出版日期Book 2016
关键词Bacteria producing esters; Isoprenoid biosynthesis; Alkanes; Recombinant rhamnolipid; Peptide antimicrob
版次1
doihttps://doi.org/10.1007/978-3-662-49127-0
isbn_softcover978-3-662-56981-8
isbn_ebook978-3-662-49127-0Series ISSN 1949-2448 Series E-ISSN 1949-2456
issn_series 1949-2448
copyrightSpringer-Verlag Berlin Heidelberg 2016
The information of publication is updating

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发表于 2025-3-21 23:01:45 | 显示全部楼层
Protocols for the Production and Analysis of Isoprenoids in Bacteria and Yeast,. Specific analytical methods are provided for isoprene, limonene, terpinolene, caryophyllene, amorphadiene, linalool, nerolidol, pinene and lycopene. We focus on yeast and . as production organisms. The protocols can be modified for other organisms and products as appropriate.
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Methods for Recombinant Rhamnolipid Production, nonpathogenic host. The most prominent genes responsible for rhamnolipid synthesis, emanating from . or . species, are already identified. We describe a method for cloning and expressing the genes . and . from . in vector pVLT33 allowing for the production of mono-rhamnolipids. Furthermore, we pres
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Purification of Peptide Antimicrobials and Thioether-Stabilized Molecules Produced In Vivo by Lantion of lanthipeptides and thioether-stabilized peptides using two well-established production platforms for . and .. These are based on the dehydration and cyclization reactions carried out by the enzymes NisB and NisC, respectively, on peptides fused to the nisin leader peptide. Furthermore, we prov
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Oxyfunctionalization of Linear Alkanes with a Biosynthetic, Self-Sufficient, Selective, and Solubleld conditions. This enzyme was the first member of a novel family of biosynthetic omega hydroxylases. Detailed protocols for A13-Red construction, purification, and optimized reaction conditions for the in vitro hydroxylation of its preferred substrate (.-octane) are described in detail into this ch
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1949-2448 quired for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environment978-3-662-56981-8978-3-662-49127-0Series ISSN 1949-2448 Series E-ISSN 1949-2456
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Víctor de Lorenzotwo core criteria (Influence and initiators; Motivation and ultimate goal) are displayed with their respective values to tick and possibly some short explanation (e.g. government influence, US Wetland Mitigation following Clean Water Act). From the latter, building on the information available, a no
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