Titlebook: Hydrocarbon and Lipid Microbiology Protocols; Microbial Quantitati Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale Book 2017 Springer-Ve

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Microbial Community Profiling: SSCP and T-RFLP Techniques,inting/profiling the diversity of microbial communities by distinction of similar-sized DNA fragments, amplified from directly extracted environmental DNA with PCR primers, hybridizing to phylogenetically conserved regions of microbial genomes. Since their introduction to microbial ecology, both met

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Clone Libraries of Ribosomal RNA Gene Sequences for Characterization of Microbial Communities, expensive and time-consuming than community fingerprinting techniques, sequence analysis of clone libraries provides a high level of phylogenetic resolution due to long read lengths generated by Sanger sequencing. In this chapter, we provide the principles and methodologies of clone library constru

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Multiplex Fluorescent Antibody Microarrays and Antibody , for Microbial and Biomarker Detection in ntal samples. An FSMI consists basically in two steps: (i) specific binding of antigens (or any recognizable analyte in a sample) to the immobilized antibodies (.) on a microarray and (ii) revealing specific antigen-antibody binding by fluorescently labelled antibodies (.). Because multiplexing immu

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Studying Protistan Communities in Hydrocarbon-Contaminated Environments,tly, much of our knowledge regarding the distribution, taxonomic diversity, and activity of protists relied on classical observation and cultivation methods. The application of molecular biological approaches which are based on the analysis of phylogenetic marker genes, such as the 18S ribosomal RNA

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1949-2448 ooting advice.Features practical laboratory guidelines to pr.This Volume presents methods for quantifying microbial populations and characterising microbial communities by extracting and analysing biomarkers such as RNA, DNA and lipids. The chapters cover a wide range of topics, including: cell sepa

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Introduction to Microbial Quantitation, Community Profiling, and Array Approaches,ive experimental design and analysis. Microbial community analysis is in a boom era and the protocols in this volume reflect the wide range of methods available for use by microbiologists to better understand microbial communities in environmental samples.

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Microbial Biomass and Community Composition Analysis Using Phospholipid Fatty Acids,as gas chromatography and mass spectroscopy. The quantitative nature of the resulting data allows for the application of statistics that authenticate microbial biomass and composition differences across an environment or between treatments.

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Denaturing Gradient Gel Electrophoresis (DGGE) for Microbial Community Analysis,ngerprint bands for sequencing. All required reagents and supplies are listed, as are the sequences of the most commonly used PCR primers for DGGE of bacterial, archaeal, and eukaryotic 16S rRNA gene amplicons.

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