Titlebook: Human T-Lymphotropic Viruses; Methods and Protocol Claudio Casoli Book 2017 Springer Science+Business Media, LLC, part of Springer Nature 2

极小

A Luciferase Functional Quantitative Assay for Measuring NF-ĸB Promoter Transactivation Mediated by vitro. Although Tax oncogenic potential needs to be further elucidated, it is well established that Tax proteins activate, among others, transcription factors of the NF-ĸB family, which are involved in immune and inflammatory responses, cell growth, apoptosis, stress responses and oncogenesis. Here

占线

Generation of a Tet-On Expression System to Study Transactivation Ability of Tax-2 describe a stable and regulated high-level expression model based on Tet-On system, to study the capacity of Tax-2 to transactivate host genes. In particular, the Jurkat Tet-On cell line suitable for evaluating the ability of Tax-2 to stimulate transactivation of a specific host gene, CCL3L1 (C-C m

幻想

locus-ceruleus

Methods for Identifying and Examining HTLV-1 HBZ Post-translational Modificationstion. Several pathogenic viruses have been shown to encode proteins with PTMs, including human T-cell leukemia virus type 1 (HTLV-1) Tax and Rex regulatory proteins. HTLV-1 basic leucine zipper protein (HBZ) was hypothesized to feature PTMs due to its functional activities and interactions with cell

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Quantification of Cell Turnover in the Bovine Leukemia Virus Modelection by the related human T-lymphotropic virus type 1 (HTLV-1). In this paper, we first provide detailed protocols to inoculate cloned BLV proviruses into sheep or cattle. We also describe methods to quantify apoptosis ex vivo and cell turnover in vivo.

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Analysis of NK Cell Function and Receptor Expression During HTLV-1 and HTLV-2 Infectioncted patients and healthy donors. Here, we described protocols to NK cells phenotypical and cytotoxicity assay, performed by flow cytometry on fresh and immune-magnetically or flow cytometry sorted NK cells. A new developed protocol able to evaluate IFNγ production has been included.

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