Titlebook: Human T-Lymphotropic Viruses; Methods and Protocol Claudio Casoli Book 2017 Springer Science+Business Media, LLC, part of Springer Nature 2

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Serological and Molecular Methods to Study Epidemiological Aspects of Human T-Cell Lymphotropic Virue main modes of HTLV-1 transmission have been described; (1) from mother-to-child after prolonged breast-feeding lasting more than six months, (2) through sexual intercourse, which mainly, but not exclusively, occurs from male to female and lastly, (3) from contaminated blood products, which contain

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Generation of a Tet-On Expression System to Study Transactivation Ability of Tax-2transferring . gene in Jurkat Tet-On cellular model and exposing transfected cells to Dox (doxycycline, a tetracycline derivate), a kinetics of .-2 expression through TaqMan Real-time PCR assay was determined.

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Expression of HTLV-1 Genes in T-Cells Using RNA Electroporationy T-cells using an approach based on the electroporation of in vitro-transcribed RNA. Results showed that the RNA transfection technique combines a high transfection efficiency with low toxicity, not only in Jurkat T-cells but also in primary T-cells. These findings suggest that RNA electroporation

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Book 2017tropic Viruses (HTLVs), featuring the most updated technical information about HTLV determination and the methods to investigate their interaction with the host immune system and interfering pathogens. The contents include essential aspects of epidemiology and virus transmission, novel and robust me

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Molecular Epidemiology Database for Sequence Management and Data Mining which allows researchers to download sequences with clinical and demographic information. The HTLV-1 Subtyping Tool is an online software used for HTLV-1 genotyping, the algorithm consists in the alignment of a query sequence with a carefully selected set of predefined reference strains, followed by phylogenetic analysis.

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Methods for Identifying and Examining HTLV-1 HBZ Post-translational Modificationsular transcription factors and acetyltransferases. Here, we describe the approach used to identify, via mass spectrometry, the PTMs of HBZ. In addition, we describe methods to determine the functional relevance of the identified PTMs.