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Titlebook: Human Retroviruses; Methods and Protocol Elisa Vicenzi,Guido Poli Book 2014 Springer Science+Business Media, LLC 2014 Accessory genes.HIV l

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SIVSM/HIV-2 Vpx Proteins: Function and Uses in the Infection of Primary Myeloid Cells the efficiency of lentiviral infection of dendritic cells (DCs), macrophages, and monocytes. Here, we describe how the positive function exerted by Vpx during the early phases of infection of myeloid cells can be used to augment the efficiency of lentiviral vector-mediated gene transfer in these cells.
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The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoatinge stability during infection. This assay is based on the biochemical separation of soluble capsid protein from particulate capsid cores and provides information about the fate of the capsid during infection.
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The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells (OMK) cells are infected with a GFP reporter virus and TRIM-CypA restriction is switched on at various times postinfection allowing the kinetics of uncoating to be monitored in infected cells. This assay also can be used to examine the effect of different viral or cellular factors on the process of uncoating.
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