Titlebook: Human Retroviruses; Methods and Protocol Elisa Vicenzi,Guido Poli Book 2014 Springer Science+Business Media, LLC 2014 Accessory genes.HIV l

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Book 2014lit into five sections, this detailed volume covers mapping of the HIV life cycle, isolation, co-receptor use, and cell tropism of HIV-1, in vivo quantification of HIV-1, biological aspects of HIV-1, as well as HTLVs. Some articles explore “assay and function of accessory genes”, largely involving t

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HIV-1 Reverse Transcription the complete viral DNA flanked by the two long-terminal repeats (LTR), required for viral DNA integration into the host genome and its expression. Here we describe assays for in vitro and ex vivo monitoring of reverse transcription and the chaperoning role of the nucleocapsid protein (NC).

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HIV-1 Accessory Proteins: VpRen proposed. In this chapter, we describe two best characterized activities of VpR, nuclear import of the HIV-1 preintegration complex (PIC) and induction of cell cycle G2 arrest, focusing on the methods used for their demonstration.

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SIVSM/HIV-2 Vpx Proteins: Function and Uses in the Infection of Primary Myeloid Cells the efficiency of lentiviral infection of dendritic cells (DCs), macrophages, and monocytes. Here, we describe how the positive function exerted by Vpx during the early phases of infection of myeloid cells can be used to augment the efficiency of lentiviral vector-mediated gene transfer in these cells.