Titlebook: Human Respiratory Syncytial Virus; Methods and Protocol Ralph A. Tripp,Patricia A. Jorquera Book 2016 Springer Science+Business Media New Y

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Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays,scribed. The first EIA uses RSV lysate antigens produced in HEp-2 cell line. The second EIA uses RSV F or G gene-expressed antigen in HEp-2 cells. The third EIA uses 30-amino acid synthetic peptides from central conserved region of G protein of RSV A2 or RSV B1 virus and a peptide from the SARS CoV

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Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection,and detecting infection. Neutralizing antibody activity is normally measured by a plaque reduction neutralization assay or by a microneutralization assay with or without complement. These assays measure the functional capacity of serum (or other fluids) to neutralize virus infectivity in cells as co

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In Vitro Modeling of RSV Infection and Cytopathogenesis in Well-Differentiated Human Primary Airwayy adapted to infection of human hosts. Rodent models, such as mice and cotton rats, are semi-permissive to RSV infection and do not faithfully reproduce hallmarks of RSV disease in humans. Furthermore, immortalized airway-derived cell lines, such as HEp-2, BEAS-2B, and A549 cells, are poorly represe

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Use of Minigenome Systems to Study RSV Transcription,e, we describe the RSV minigenome assay for determining transcription by the viral polymerase in the absence of infection. We detail two different methods of detecting viral RNA synthesis: a firefly luciferase assay for rapid and sensitive measurement of RSV polymerase activity; and a real-time quan

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Screening for Host Factors Directly Interacting with RSV Protein: Microfluidics,in. The device consists of thousands of reaction chambers controlled by micro-mechanical valves. The microfluidic device is mated to a microarray-printed custom-made gene library. These genes are then transcribed and translated on-chip, resulting in a protein array ready for binding to RSV M protein

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A Proteomic-Based Workflow Using Purified Respiratory Syncytial Virus Particles to Identify Cellulaion of druggable cellular protein that are essential for RSV replication. In this regard experimental strategies that are able to screen relevant proteins from the vast array of proteins in the cellular milieu will facilitate the identification of potential drug targets. In this chapter we describe