Titlebook: Human Respiratory Syncytial Virus; Methods and Protocol Ralph A. Tripp,Patricia A. Jorquera Book 2016 Springer Science+Business Media New Y

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Reverse Genetics of Respiratory Syncytial Virus,rently no available vaccines. Here, we describe the methods for recovery of recombinant RSV viruses using a bacterial artificial chromosome and methods related to procurement and expansion of stocks of RSV mutants.

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Use of Minigenome Systems to Study RSV Transcription,hods of detecting viral RNA synthesis: a firefly luciferase assay for rapid and sensitive measurement of RSV polymerase activity; and a real-time quantitative PCR method for determination of specific effects on the transcription of individual viral genes and the polar transcription gradient of RSV.

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Mouse and Cotton Rat Models of Human Respiratory Syncytial Virus,ity and mortality rates especially if there are underlying health issues. Despite many years of effort, no vaccine or specific treatments exist and RSV is still the leading cause of infant hospitalizations worldwide. Here, we describe methods to infect two widely used small animal models: laboratory mice and cotton rats.

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Secretory Expression and Purification of Respiratory Syncytial Virus G and F Proteins in Human Cell disease and for developing vaccines and access to purified, expressed proteins is important to RSV research and diagnostics. We describe methods to produce recombinant RSV F and G proteins in human cells and purify these proteins using Ni Sepharose affinity chromatography.

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Development of Human Monoclonal Antibodies Against Respiratory Syncytial Virus Using a High Efficieyeloma cells by dielectrophoresis to generate human hybridomas secreting RSV-specific monoclonal antibodies. We also provide experimental methods for screening human B cell lines to obtain RSV-specific lines, especially lines secreting neutralizing antibodies.