Titlebook: Human Fungal Pathogen Identification; Methods and Protocol Thomas Lion Book 2017 Springer Science+Business Media New York 2017 Invasive fun

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Serological Approachesn delayed. Non-culture-based methods with higher sensitivity and specificity may reduce diagnostic time and result in decreased IFD morbidity and mortality. These methods are now increasingly used to manage patients at risk of IFD. Among available biomarkers, fungal antigens have been investigated a

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Isolation of Nucleic Acids for Fungal Diagnosisiding robustness, they make it difficult to identify optimal strategies, limiting standardization, and widespread acceptance. No matter how efficient, PCR utility will be limited by the quality of extracted nucleic acid. This chapter highlights benefits and limitations affecting the nucleic acid ext

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Prerequisites for Control of Contamination in Fungal Diagnosisty of patient specimens. However, contamination from various exogenous sources constitutes a serious threat to the validity of amplification-based fungal assays. In this chapter, common origins of fungal contaminants that compromise molecular fungal testing are described, and measures for preventing

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Genus- and Species-Specific PCR Detection Methodsrent clinical specimen types can be used as original material. However, certain precautions, in addition to the published MIQE guidelines [.], need to be taken to prevent contaminations from airborne fungal spores and PCR reagents. In addition, the European . PCR Initiative (EAPCRI) recently defined

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Identification of Fungal Pathogens in Tissue Samples from Patients with Proven Invasive Infection byasive fungal infections. Rapid and specific fungal identification has been documented in different diagnostic settings allowing for specific patient management. Identification of fungal pathogens in formalin-fixed, paraffin-embedded tissue samples appears to be rewarding as these materials are store

摘要

The Changing Epidemiology of Invasive Fungal Infectionsother disease conditions and the ecological niche for the organism; for instance cryptococcal and pneumocystis infections are particularly common in areas with a high prevalence of HIV disease. Poorly controlled diabetes is a major risk factor for invasive mould infections. Environmental factors and