Titlebook: Human Cell Culture Protocols; Joanna Picot Book 2005Latest edition Humana Press 2005 genes.phorbol ester.regulation.reproducible technique

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1543-1894 aboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.978-1-61737-431-9978-1-59259-861-8Series ISSN 1543-1894 Series E-ISSN 1940-6037

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Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters,cts on multiple cellular responses (.). Recent progress in basic cell-culture technology, along with an improved understanding of culture requirements, has led to an effective and standardized isolation method, and special TPA-free culture media for selective growth and long-term maintenance of huma

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Human Myoblasts and Muscle-Derived SP Cells,erentiationspecific proteins. In this chapter, methods are given for the isolation of myoblasts from human muscle tissue using two different techniques: (a) flow cytometry (.) and (b) cell cloning (.,.). Recent reports have also highlighted the existence of highly primitive cells within mouse skelet

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Primary Culture and Differentiation of Human Adipocyte Precursor Cells,rleukin 6 (IL-6), as well as angiotensinogen and plasminogen activator inhibitor-1 (PAI-1) are some of the products secreted by adipocytes (.-.). When the balance between energy intake and expenditure is such that the intake exceeds the need of the body, adipose tissue expands and the individual bec

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Human Fetal Brain Cell Culture,lack of an alternative source of viable normal human brain cells. Although research that employs human fetal tissue has been limited, what has been done has made a major impact in the development of prophylactics and therapeutics for several important human diseases. For example, the use of fetal ti

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A New Approach to Primary Culture of Human Gastric Epithelium,wo different approaches, i.e., microsurgi-cal/scraping techniques and enzymatic treatments. The first requires laboratory expertise and, unless gradient fractionation or elutriation is used to purify glandular cells, these are rapidly overgrown during the first 2 d of culture by dividing mucous cell