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Titlebook: Human Cell Culture Protocols; Joanna Picot Book 2005Latest edition Humana Press 2005 genes.phorbol ester.regulation.reproducible technique

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发表于 2025-3-21 17:42:36 | 显示全部楼层 |阅读模式
书目名称Human Cell Culture Protocols
编辑Joanna Picot
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Medicine
图书封面Titlebook: Human Cell Culture Protocols;  Joanna Picot Book 2005Latest edition Humana Press 2005 genes.phorbol ester.regulation.reproducible technique
描述A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
出版日期Book 2005Latest edition
关键词genes; phorbol ester; regulation; reproducible techniques; tissue
版次2
doihttps://doi.org/10.1385/1592598617
isbn_softcover978-1-61737-431-9
isbn_ebook978-1-59259-861-8Series ISSN 1543-1894 Series E-ISSN 1940-6037
issn_series 1543-1894
copyrightHumana Press 2005
The information of publication is updating

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Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules,The kidney is an extremely heterogeneous organ from a morphological and a functional point of view. In spite of this heterogeneity, several methods have been described in an attempt to isolate and culture homogeneous populations of renal epithelial cells, seeking to maintain normal differentiated characteristics of the nephron segment of origin.
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Joanna PicotIncludes supplementary material:
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Human Cell Culture Protocols978-1-59259-861-8Series ISSN 1543-1894 Series E-ISSN 1940-6037
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Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters,idermal melanocytes has been attempted since 1957 (.-.), but only since 1982 have pure normal human melanocyte cultures been reproducibly established to yield cells in sufficient quantity for biological, biochemical, and molecular analyses (.). Selective growth of melanocytes, which comprise only 3-
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Isolation and Culture of Human Osteoblasts,dal cell rich in organelles involved in the synthesis and secretion of matrix proteins. (.) Osteocyte-an osteoblast with low metabolic activity that has been engulfed in matrix during bone formation and entombed in lacunae. (.) Bone-lining cell-osteoblasts that have avoided entombment in lacunae and
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Human Osteoclast Culture From Peripheral Blood Monocytes,cells and, therefore, they cannot simply divide and be maintained in culturean osteoclast supply must constantly be replenished. Osteoclasts are large, multinucleated cells formed by the fusion of osteoclast precursors found in the monocyte fraction of blood. The osteoclast is thought to be the only
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