Titlebook: Histopathology; Methods and Protocol Christina E. Day Book 2014 Springer Science+Business Media New York 2014 diagnosis.diseases.histopatho

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The Pathologist’s Guide to Fixativesclinical counterparts regarding what fixative should be used for different tissues or to enable a diagnosis of a specific condition. It is vital for the patient that the pathologist provides accurate information to ensure proper fixation. Frequently, once a tissue has been fixed inadequately or inap

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Tissue Processing and Hematoxylin and Eosin Stainingcytoplasm contrasting colors to readily differentiate cellular components. However, staining results are dependent on proper specimen processing, which involves tissue preservation, dehydration, clearing, and paraffin infiltration. While improvements in instrumentation for both tissue processing and

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Light Microscope Alignment Methodsn, dark field, phase contrast, differential interference contrast, and fluorescence. These are then followed by methods on photomicrography, measurement, and cleaning. An extensive set of Notes provides practical tips for each of these methods.

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Optimizing Direct Immunofluorescencetibodies used in a clinical laboratory bind the conserved domains within each class of human antibodies, allowing them to detect a wide range of autoimmune complexes. Drawbacks to this technique mostly relate to proper handling of the specimen and the fluorophore-labeled antibodies. Therefore, havin

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Electron Microscopy: A Brief History and Review of Current Clinical Applicationmicroscopy, for diagnostic purposes. It was described how the standard techniques for preparing tissue for light microscopy had been modified to make it possible to view the ultrastructural components of a cell, tissue, or organ that cannot be resolved with a light microscope. There is a discussion

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A Practical Approach to Clinical and Research Biobankinganaged biospecimens. Reflecting advancements in biospecimen science, the National Cancer Institute and the International Society for Biological and Environmental Repositories are periodically publishing best practices that can guide the biobanker. The modern biobank will operate more like a clinical

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Hospital Autopsy Performance and Reportinger Arnold, Hachette UK, London, pp 1–10, 2010) and have a long and venerable tradition, one which is sadly on the decline worldwide (Underwood, The future of the autopsy. Hodder Arnold, Hachette UK, London, pp 11–17, 2010; Nemetz et al., Mayo Clin Proc 64:1065–2076, 1989; Kaplan, Hum Pathol 9(2):127

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Intraoperative Frozen Section Consultation in Gynecologic Pathology extent of disease in order to assist the surgeon in determining the surgical procedure being performed. This chapter discusses the main indications for IOC in gynecologic pathology and describes how to submit and process these specimens in order to obtain optimal material. In addition, diagnostic p