Titlebook: Histone Variants; Methods and Protocol Guillermo A. Orsi,Geneviève Almouzni Book 2018 Springer Science+Business Media, LLC, part of Springe

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Characterization of Posttranslational Modifications on Histone Variantsvariants expressed in bacteria. In addition, we describe a Triton-Acetic acid-Urea (TAU) gel electrophoresis protocol adapted to work on mini-gels, which can be coupled to Western blot to analyze PTMs on histone variants. Finally, we describe a Chromatin immunoprecipitation (ChIP) assay for studying

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Imaging Newly Synthesized and Old Histone Variant Dynamics Dependent on Chaperones Using the SNAP-Taerritories. Here, we provide a detailed protocol to exploit the SNAP-tag technology to assess the dynamics of newly synthesized and old histones. We then show that combining the SNAP-tagging of histones with the knockdown of candidate factors, represents an effective approach to decipher the role of

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CRISPR/Cas9 Gene Editing of Human Histone H2A Variant H2AX and MacroH2A2AX and macroH2A histone H2A variants using CRISPR/Cas9 gene editing in human cancer cell lines. This protocol allows the use and development of genetic systems in human cells to study histone variants and their functions, including within the DDR.

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Tomoya Kujirai,Yasuhiro Arimura,Risa Fujita,Naoki Horikoshi,Shinichi Machida,Hitoshi Kurumizaka