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Titlebook: Histone Variants; Methods and Protocol Guillermo A. Orsi,Geneviève Almouzni Book 2018 Springer Science+Business Media, LLC, part of Springe

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发表于 2025-3-21 19:34:59 | 显示全部楼层 |阅读模式
书目名称Histone Variants
副标题Methods and Protocol
编辑Guillermo A. Orsi,Geneviève Almouzni
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Histone Variants; Methods and Protocol Guillermo A. Orsi,Geneviève Almouzni Book 2018 Springer Science+Business Media, LLC, part of Springe
描述.This volume compiles methodologies used for detailed studies of histone variants, from their basic properties to their functional roles in chromatin and as vectors of epigenetic information. Its four sections cover experimental approaches to probe the biochemistry of histone variants and variant nucleosomes; their dynamics throughout the cell cycle and at specific genomic locations; their functional impact on chromosome organization and genome stability; and their importance in development, disease and evolution. Written in the highly successful .Methods in Molecular Biology. series format, chapters include lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, illustrations on the expected results, and tips on troubleshooting and avoiding known pitfalls. This anthology brings together contributions from many of the most highly recognized investigators in this community..Cutting-edge and thorough, .Histone Variants: Methods and Protocols. is a valuable resource for anyone interested in exploring the experimental and collaborative possibilities in this evolving field..
出版日期Book 2018
关键词chromatin integrity; nucleosomes; histone chaperones; cellular functions; epigenomics
版次1
doihttps://doi.org/10.1007/978-1-4939-8663-7
isbn_softcover978-1-4939-9358-1
isbn_ebook978-1-4939-8663-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2018
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Characterization of Posttranslational Modifications on Histone Variantsgy, neurobiology, and immunology; as well as in the understanding of molecular mechanisms leading to diverse diseases, such as cancer. Since the establishment of histone PTMs starts immediately after their synthesis and it continues once they are assembled into chromatin, here we describe a classic
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Purification of Histone Variant-Interacting Chaperone Complexesrotein in cells. The pre-deposited forms of histones are associated with the specific histone chaperones to assemble into chromatin. Here, I describe an affinity purification method using the FLAG/HA double epitope-tagging technique and its application to purify particular histone variant-interactin
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How to Tackle Challenging ChIP-Seq, with Long-Range Cross-Linking, Using ATRX as an Examplewide patterns of chromatin modifications and binding of chromatin-associated proteins. Well-established protocols produce robust ChIP-seq data for many proteins by sequencing the DNA obtained following immunoprecipitation of fragmented chromatin using a wide range of specific antibodies. In general,
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