Titlebook: High-Throughput Protein Production and Purification; Methods and Protocol Renaud Vincentelli Book 2019Latest edition Springer Science+Busin

没花的是打扰

978-1-4939-9626-1Springer Science+Business Media, LLC, part of Springer Nature 2019

悲观

陈腐的人

Dealing

Overview of a High-Throughput Pipeline for Streamlining the Production of Recombinant ProteinsEmbryonic Kidney (HEK) cells is used exclusively for production of secreted glycoproteins. Protein purification and quality assessment are independent of the expression system and enable sample preparation to be simplified and streamlined.

Compass

Spinal-Tap

High-Throughput Site-Directed Mutagenesis work. When dealing with large volumes of samples it is not possible to use such a low-throughput approach. Here we describe a high-throughput (HTP) method for SDM, optimized and used by the Structural Genomics Consortium (SGC) to complement structural studies.

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High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a Muln the M1 capsid protein from influenza, and its application to produce in a parallelized fashion an array of influenza-derived VLPs containing functional mutations in influenza hemagglutinin (HA) thought to modulate the immune response elicited by the VLP.

疏远天际

High-Throughput Protein Production of Membrane Proteins in smid. These membrane proteins are then screened for expression level, detergent solubilization, initial purity, and chromatography characteristics. Both small- and large-scale expression methods are discussed along with fermentation.

BLANC

languid

Book 2019Latest editioned over the years and are now ready to be transferred to any laboratory. Beginning with a section covering general procedures for high-throughput protein production, the volume continues with high-throughput protocols adapted to the production of specific protein families, as well as an extensive se