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Titlebook: High-Throughput Protein Production and Purification; Methods and Protocol Renaud Vincentelli Book 2019Latest edition Springer Science+Busin

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发表于 2025-3-21 19:58:39 | 显示全部楼层 |阅读模式
书目名称High-Throughput Protein Production and Purification
副标题Methods and Protocol
编辑Renaud Vincentelli
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: High-Throughput Protein Production and Purification; Methods and Protocol Renaud Vincentelli Book 2019Latest edition Springer Science+Busin
描述This book compiles key protocols instrumental to the study of high-throughput protein production and purification which have been refined and simplified over the years and are now ready to be transferred to any laboratory. Beginning with a section covering general procedures for high-throughput protein production, the volume continues with high-throughput protocols adapted to the production of specific protein families, as well as an extensive section on protocols combining high-throughput protein production and their micro-characterization. Written for the highly successful .Methods in Molecular Biology. series, chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .High-Throughput Protein Production and Purification: Methods and Protocols. serves biochemists ranging from engineers, PhD students and post-doctoral fellows, to the heads of protein expression facilities and researchers, in pursuing this vital area of study..
出版日期Book 2019Latest edition
关键词Protein expression; Micro-characterization; Up-scaling production; Soluble yield; Structural genomics
版次2
doihttps://doi.org/10.1007/978-1-4939-9624-7
isbn_softcover978-1-4939-9626-1
isbn_ebook978-1-4939-9624-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

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Overview of a High-Throughput Pipeline for Streamlining the Production of Recombinant Proteins use of streamlined workflows with standard operating procedures and automation. In this chapter, we describe the Oxford Protein Production Facility (OPPF) pipeline for protein production, from conception, through vector construction, to expression and purification. Results from projects run in the
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A High-Throughput System for Transient and Stable Protein Production in Mammalian Cellstory robotics have enabled the development of highly parallel and rapid processes for cell culture and protein expression, purification, and analysis. Human embryonic kidney (HEK) cells and Chinese hamster ovary (CHO) cells have emerged as the standard host cell workhorses for producing recombinant
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High-Throughput Production of Oxidized Animal Toxins in -genomic information currently available from various sources. One such source is animal venom, which contains thousands of novel bioactive peptides with potential uses as novel therapeutics to treat a plethora of diseases as well as in environmentally benign bioinsecticide formulations. Here, we de
发表于 2025-3-23 01:08:44 | 显示全部楼层
High-Throughput Purification of Protein Kinases from , and Insect Cells, posttranslational modifications, and the large size of some protein kinases pose a particular challenge obtaining homogeneous, active recombinant protein kinases suitable for functional or structural studies. Here we describe our expertise expressing protein kinases in two frequently used host sys
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High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a Mulplications ranging from multiprotein complex structural biology to manufacturing of therapeutic proteins including virus-like particles (VLPs). VLPs are protein assemblies that mimic live viruses but typically do not contain any genetic material, and therefore are safe and attractive alternatives to
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