Titlebook: High-Throughput Metabolomics; Methods and Protocol Angelo D‘Alessandro Book 2019 Springer Science+Business Media, LLC, part of Springer Nat

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Traveling Wave Ion Mobility Mass Spectrometry: Metabolomics Applicationsies have been integrated with mass spectrometry (MS) and launched as commercially available instrumentation for metabolomics analysis. The addition of IM to MS-based metabolomics workflows provides an additional degree of separation to chromatography and MS resolving power, improving peak capacity a

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Overview of Lipid Mass Spectrometry and Lipidomicsemerged in order to carry out lipidomic studies. These include both shotgun approaches as well as those engaging liquid chromatographic separation of lipid species prior to mass spectrometric analysis. Nonetheless challenges remain at every level of the lipidomic experiment, including extraction of

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LC-MS/MS-MRM-Based Targeted Metabolomics for Quantitative Analysis of Polyunsaturated Fatty Acids an metabolites. Given the complexity of biological samples, the difference in ionization efficiency, and signal intensity of each metabolite, isotopically labeled internal standards are often used for accurate quantification. In this chapter, we describe a detailed protocol for the quantitative analys

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Untargeted and Semi-targeted Lipid Analysis of Biological Samples Using Mass Spectrometry-Based Metaly study biological systems. Historically, unbiased (or discovery) analysis has been performed independently of targeted, quantitative analysis such as multiple reaction monitoring (MRM). These practices have been aptly carried out based on technical limitations of each assay. The wide mass scanning

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HPLC-MS/MS Methods for Diacylglycerol and Sphingolipid Molecular Species in Skeletal Muscleen optimized for individual lipid classes, provide good lipidomic profiles, but may not be desirable for laboratories constrained by available instrumentation and wanting a higher throughput. Here we describe two methods using binary gradient HiLiC HPLC and triple quadrupole MS that together provide

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