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Titlebook: High-Throughput Metabolomics; Methods and Protocol Angelo D‘Alessandro Book 2019 Springer Science+Business Media, LLC, part of Springer Nat

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书目名称High-Throughput Metabolomics
副标题Methods and Protocol
编辑Angelo D‘Alessandro
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: High-Throughput Metabolomics; Methods and Protocol Angelo D‘Alessandro Book 2019 Springer Science+Business Media, LLC, part of Springer Nat
描述This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracers, in biological matrices of clinical relevance and model organisms. Mass spectrometry-based or orthogonal methods are discussed, along with computational and statistical methods to address data sparsity in high-throughput metabolomics approaches. As a part of the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .High-Throughput Metabolomics: Methods and Protocols. provides tools that can bring about thenext generation of clinical biochemistry in a cost-effective, rigorous fashion, exponentially advancing our capacity to investigate nature while hastening the advent of personalized medicine..
出版日期Book 2019
关键词Mass Spectrometry; Data processing; Lipidomics; Biostatistics; Personalized medicine; Precision medicine;
版次1
doihttps://doi.org/10.1007/978-1-4939-9236-2
isbn_softcover978-1-4939-9238-6
isbn_ebook978-1-4939-9236-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
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High-Throughput Metabolomics: Isocratic and Gradient Mass Spectrometry-Based Methodsites measured in the 3 min run, the use of the longer gradient run here also allows for coverage of less polar compounds such as fatty acids and acylcarnitines, both key players in mitochondrial and lipid metabolism, without a significant sacrifice in throughput.
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Overview of Lipid Mass Spectrometry and Lipidomicson species to that of an internal standard enables quite accurate assessment of fold changes within complex lipid species without the need for exact quantitation. Challenges continue to remain in terms of availability of reference standard material as well as relevant internal standards.
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Untargeted and Semi-targeted Lipid Analysis of Biological Samples Using Mass Spectrometry-Based Metaus making these instruments more robust. By combining the improved robustness and coverage with stable isotope dilution (SID) techniques, advantages of the separate assays can now be realized in a single run, thereby improving the throughput of this type of analysis.
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1064-3745 expertsThis detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labele
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