书目名称 | High Throughput Screening |
副标题 | Methods and Protocol |
编辑 | William P. Janzen |
视频video | http://file.papertrans.cn/427/426536/426536.mp4 |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .This third edition volumeexpands on the previous editions both by presenting more detailed protocols forthe techniques described in the first and second editions of .High ThroughputScreening: Methods and Protocols.and by covering important new procedures... The first chapter ofthis book provides an overview of important assay development techniques, whilethe rest of the chapters detail how to develop and execute screens at whateverthroughput the user needs. Some chapter examples are: structure-based virtualscreening, high throughput screening using mass spectrometry, identification of state-dependentblockers for voltage gated calcium channels, bioluminescence resonanceenergy transfer platform to monitor protein-protein interactions in live cells,high throughput flow cytometry, and application of imaging-based assays in microplate formats for highcontent screening. Written in the highly successful .Methods in MolecularBiology .series format, chaptersinclude introductions to their respectivetopics, lists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls..Cutting edge and thorough, |
出版日期 | Book 2016Latest edition |
关键词 | high throughput screening assays; complex cellular systems; mass spectrometer techniques; drug discover |
版次 | 3 |
doi | https://doi.org/10.1007/978-1-4939-3673-1 |
isbn_softcover | 978-1-4939-8113-7 |
isbn_ebook | 978-1-4939-3673-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media New York 2016 |