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Titlebook: High Throughput Screening; Methods and Protocol William P. Janzen Book 2016Latest edition Springer Science+Business Media New York 2016 hig

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发表于 2025-3-21 18:47:09 | 显示全部楼层 |阅读模式
书目名称High Throughput Screening
副标题Methods and Protocol
编辑William P. Janzen
视频videohttp://file.papertrans.cn/427/426536/426536.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: High Throughput Screening; Methods and Protocol William P. Janzen Book 2016Latest edition Springer Science+Business Media New York 2016 hig
描述.This third edition volumeexpands on the previous editions both by presenting more detailed protocols forthe techniques described in the first and second editions of .High ThroughputScreening: Methods and Protocols.and by covering important new procedures... The first chapter ofthis book provides an overview of important assay development techniques, whilethe rest of the chapters detail how to develop and execute screens at whateverthroughput the user needs. Some chapter examples are: structure-based virtualscreening,  high throughput screening using mass spectrometry, identification of state-dependentblockers for voltage gated calcium channels, bioluminescence resonanceenergy transfer platform to monitor protein-protein interactions in live cells,high throughput flow cytometry, and application of imaging-based assays in microplate formats for highcontent screening. Written in the highly successful .Methods in MolecularBiology .series format, chaptersinclude introductions to their respectivetopics, lists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls..Cutting edge and thorough,
出版日期Book 2016Latest edition
关键词high throughput screening assays; complex cellular systems; mass spectrometer techniques; drug discover
版次3
doihttps://doi.org/10.1007/978-1-4939-3673-1
isbn_softcover978-1-4939-8113-7
isbn_ebook978-1-4939-3673-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
The information of publication is updating

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发表于 2025-3-21 20:39:39 | 显示全部楼层
Design and Implementation of High-Throughput Screening Assays, be made that can greatly impact the outcome of the HTS effort, to the point of making it a success or a failure. Although specific guidelines should be established to ensure that the screening assay reaches an acceptable level of quality, many choices require pragmatism and the ability to compromise opposing forces.
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Instrument Quality Control,id dispensers. Two techniques, gravimetric QC and photometric QC, are highlighted in this chapter. When used together, these simple techniques provide a robust process for evaluating liquid handler accuracy and precision, and critically underpin high-quality research programs.
发表于 2025-3-22 09:48:30 | 显示全部楼层
, High-Throughput Screening,cteria to use because of its slow growth and assignment to Biosafety Level-3 by the CDC and NIH. The principles illuminated here, however, are relevant to the execution of most bacteria high-throughput screens.
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A Fluorescence-Based High-Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenibrary against the clinically relevant but less frequently screened fungal pathogen .. This enables the user to conduct high-throughput screening using a disease-relevant fungal growth assay and identify novel antifungal chemotypes as drug leads.
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1064-3745 ation advice from the experts.This third edition volumeexpands on the previous editions both by presenting more detailed protocols forthe techniques described in the first and second editions of .High ThroughputScreening: Methods and Protocols.and by covering important new procedures... The first ch
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