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Titlebook: High Content Screening; D. Lansing Taylor,Jeffrey R. Haskins,Kenneth A. Gi Book 2006 Humana Press 2006 bioinformatics.biology.cell biology

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The HaloTag™ions and dynamics in living cells. Here, we describe a technology for covalent tethering of organic probes directly to a specially designed reporting protein expressed in live cells. The reporting protein can be used in a manner similar to green fluorescent protein, except that the fluorophore might
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Protein Labeling With FlAsH and ReAsHall molecule detection reagent makes this technology particularly suitable for the investigation of biochemical changes in living cells that are difficult to approach with fluorescent proteins as molecular tags. We describe the practical application of this technology to image protein dynamics in living cells.
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Book 2006 research has been primarily focused on hypothesis-driven research. However, the success of the human genome project, a discovery-driven research approach, has opened the door to adding other types of discovery-driven research to a continuum of research approaches. In contrast, drug discovery resear
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