Titlebook: Herpes Simplex Virus Protocols; S. Moira Brown,Alasdair R. MacLean Book 1998 Humana Press 1998

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HSV Growth, Preparation, and Assay,quire the virus to be grown and assayed. The following chapter is therefore seen as the fundamental first step before embarking on more intellectually and technically challenging technology. Its importance should not however be underestimated. It never fails to surprize us that people who describe t

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HSV Mutagenesis,begin as the virus enters the cell, and continue as the decision for latency or lytic replication is made. Correctly regulated gene expression then allows herpes genes to be expressed in a temporally regulated manner and to subvert the host cell metabolism in favor of virus production. Finally, infe

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Saturating Mutagenesis and Characterization of a Herpesvirus Genome Using In Vivo Reconstitution ofs. In this respect, mutagenesis and sequence analysis of genes encoded by the virus are of great importance. However, the herpesvirus genomes are large, with sizes ranging between 120 and over 200 kbp and encoding between 70 and 200 genes (. ref. . for a review). This large size hampers handling and

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Construction and Use of Cell Lines Expressing HSV Genes, in strategies for the construction of mutant viruses. More recent applications include the propagation of replication-defective virus products with potential as viral vaccines (.,.) or as vehicles for gene therapy (.,.). The perceived requirements for such systems are low recombination frequencies

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Analysis of HSV Polypeptides,roduced, including a host shut off protein (.–.). The inhibitory effect of the protein obviously needs a certain period of time to be fully efficient, but some cellular proteins involved in virus replication still are produced. Examples are those interacting with the regulatory viral proteins V.65 (

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Protein Purification,a protein can be used to generate specific polyclonal and monoclonal antibodies for in vivo studies, in vitro the enzymic properties or interactions with nucleic acids or other proteins can be studied in detail and related to in viva function and, ultimately, the purified proteins can be used in str

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Expression and Purification of Secreted Forms of HSV Glycoproteins from Baculovirus-Infected Insectonatal infections. Most adults have antibodies against the oral form of the virus HSV-1 (.), and a significant number are infected with the genital form, HSV-2. Both serotypes establish lifelong latent infections and reactivate periodically to produce recurrent disease (.). After infection, virus-en

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Crystallization of Macromolecules for Three-Dimensional Structure Determination,ation, and has been made possible by the combined improvement in techniques of X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy, and the bulk preparation of biological materials. Most crucial, however, has been the desire of the experimental biologists to follow research problems