书目名称 | Hepatitis B Virus |
副标题 | Methods and Protocol |
编辑 | Haitao Guo,Andrea Cuconati |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .This volume serves as a reference for the dissemination of advances made in the study of Hepatitis B Virus (HBV). .Hepatitis B Virus: Methods and Protocols. details protocols and techniques ranging from cell culture studies to in vivo and clinical immunology. The chapters in this book discuss treatments of in vitro infection systems, analysis and quantification of cccDNA and its mutations; in vitro polymerase activity assays; cellular trafficking of core proteins; intracellular calcium metabolism; detection, cloning, and sequencing of HBV markers; and new strategies aimed at exploiting new mechanisms for drug discovery. The book also covers classical methods for resolution of extracellular viral particles by native gel electrophoresis, and methods for detecting HBV antigens in drug discovery. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and comprehensive, .Hepatitis B Virus: Methods and Protocols. is a valuable tool fo |
出版日期 | Book 2017 |
关键词 | DNA and RNA viruses; covalently closed circular (ccc) DNA; genome; immunotolerance; in vitro polymerase |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-4939-6700-1 |
isbn_softcover | 978-1-4939-8277-6 |
isbn_ebook | 978-1-4939-6700-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media LLC 2017 |