Titlebook: Hemostasis and Thrombosis Protocols; David J. Perry,K. J. Pasi Book 1999 Springer Science+Business Media New York 1999

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Direct Sequencing of PCR ProductsSequencing is an increasingly important part of the analysis of a polymerase chain reaction (PCR) product and is used in many areas of hemostasis. The protocols described in this chapter have been used to sequence many different PCR templates and provide high-quality, reproducible sequence data.

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Inversion Mutation Analysis in Hemophilia A by Restriction Enzyme Analysis and Southern BlottingIntron 22 of the factor VIII gene contains a 9.5kb region of DNA that is repeated on at least two other locations telomeric to, and at least 500 kb from, the gene. These regions are termed intron 22 homologous regions (int22h) (.) and contain the Factor VIII associated gene (F8A).

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Methods in Molecular Medicinehttp://image.papertrans.cn/h/image/425624.jpg

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Isolation of DNA and RNAmples for DNA isolation can be stored at −50°C and the DNA prepared at a later stage. A more convenient method requiring less freezer space is to store buffy coats—the interface between the red cells and the plasma that is seen following centrifugation of whole blood. This latter method also allows isolation of the plasma fraction.

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Hemostasis and Thrombosis Protocols978-1-59259-248-7Series ISSN 1543-1894 Series E-ISSN 1940-6037