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Titlebook: Hemostasis and Thrombosis Protocols; David J. Perry,K. J. Pasi Book 1999 Springer Science+Business Media New York 1999

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书目名称Hemostasis and Thrombosis Protocols
编辑David J. Perry,K. J. Pasi
视频video
丛书名称Methods in Molecular Medicine
图书封面Titlebook: Hemostasis and Thrombosis Protocols;  David J. Perry,K. J. Pasi Book 1999 Springer Science+Business Media New York 1999
描述Laboratory studies in hemostasis have traditionally focused on abn- malities of platelet function or the quantitative and qualitative disorders that affect the proteins involved in blood coagulation. However, over the last 10 years there has been an explosion in our understanding of the molecular bases that underlie many of the inherited and acquired disorders of hemostasis. Many of these disorders are now routinely diagnosed and assessed by methods that involve genotypic analysis. Indeed in the late 1990s the distinction between molecular methods for research and for routine diagnosis is becoming incre- ingly blurred. The techniques and approaches that are used in hemostasis are manifold and published in isolation in a variety of publications. The aim, therefore, of this volume Hemostasis and Thrombosis Protocols is to pull together, into a single volume, the variety of techniques that are frequently used in the field of hemostasis. We have targeted this volume at laboratories who wish to move into the field of molecular hemostasis or who may already have some expe- ence in this area but wish to develop new areas of research and diagnosis. The chapters are wide-ranging and hopeful
出版日期Book 1999
版次1
doihttps://doi.org/10.1385/1592592481
isbn_softcover978-1-4899-4313-2
isbn_ebook978-1-59259-248-7Series ISSN 1543-1894 Series E-ISSN 1940-6037
issn_series 1543-1894
copyrightSpringer Science+Business Media New York 1999
The information of publication is updating

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Amplification of DNA and RNA by PCRof the chapters employ the technique at some point to amplify specific DNA or RNA sequences. This chapter, therefore, provides a brief outline of the techniques and methods for the amplification of both DNA and RNA. It should be remembered that no single protocol will be suitable for all PCR reactio
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Solid-Phase Sequencing of Biotinylated PCR Products with Streptavidin-Coated Magnetic Beadsf the amplification primers and as a result becomes incorporated into PCR product during the amplification reaction. The DNA can then be immobilized onto streptavidin-coated paramagnetic beads, simultaneously removing buffers, dNTPs, and unincorporated PCR primers. By alkali denaturation, the immobi
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Detection of DNA by Silver Stainingtages over conventional staining methods, including the ability to detect very small amounts of material, while avoiding the hazards of other detection systems, such as ethidium bromide or radio-isotopes.
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Promoter Studies in Hemostasised the production of cloned genomic material encompassing the putative promoter regions of genes involved in the hemostatic process. These recombinant vectors provide the raw material for reporter gene studies and DNA footprinting analysis; two of the three most frequently used in vitro procedures t
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Detection of Mutations and Polymorphisms in Clotting Factors by Denaturing Gradient Gel Electrophoretions and polymorphisms. The most frequently used screening methods for mutation hunting in bleeding disorders are denaturing gradient gel electrophoresis (DGGE) (.), single-strand conformation polymorphism (SSCP) (.), and chemical mismatch cleavage (.). This chapter gives an introduction to the the
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