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Titlebook: Hematopoietic Stem Cell Protocols; Kevin D. Bunting Book 2008Latest edition Humana Press 2008 BAC.Flow cytometry.HSC.Lentiviral transducti

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发表于 2025-3-21 18:21:37 | 显示全部楼层 |阅读模式
书目名称Hematopoietic Stem Cell Protocols
编辑Kevin D. Bunting
视频video
概述Provides detailed flow cytometry protocols for thorough analysis of enriched HSC populations.Offers a variety of transplantation approaches to measure HSC function in vivo.Details retroviral and lenti
丛书名称Methods in Molecular Biology
图书封面Titlebook: Hematopoietic Stem Cell Protocols;  Kevin D. Bunting Book 2008Latest edition Humana Press 2008 BAC.Flow cytometry.HSC.Lentiviral transducti
描述The hematopoietic stem cell (HSC) field has rapidly grown in the past several years as new technologies have been developed and the older tried and true methods have been used in new ways. Major advances in the isolation of HSC and progenitor subsets have been sparked by the gene expression array technologies, leading to the identification of new markers for prospective isolation. The first edition of Hematopoietic Stem Cell Protocols is a very thorough resource covering historically the basic techniques that have been published extensively over many years. To put together, a follow-up to this excellent book has been a hard act to follow. The intent of this book is to be distinct and not duplicate but to build on the strong basic assays and include updated approaches for the combination of phenotypic and functional analyses. This second edition of Hematopoietic Stem Cell Protocols thus aims to provide timely protocols developed in the HSC field since the last comprehensive review in this series. The approaches described in this book should be readily useful for the basic science researcher, especially those familiar with the use of mouse genetic models. The chapters in this book ar
出版日期Book 2008Latest edition
关键词BAC; Flow cytometry; HSC; Lentiviral transduction; Migration; Retroviral transduction; Stem cell investiga
版次2
doihttps://doi.org/10.1007/978-1-59745-182-6
isbn_softcover978-1-61737-816-4
isbn_ebook978-1-59745-182-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2008
The information of publication is updating

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发表于 2025-3-21 21:12:42 | 显示全部楼层
Isolation and Functional Characterization of Side Population Stem Cellshly homogeneous content of hematopoietic stem cells (HSCs). In this chapter, we describe a protocol to reproducibly isolate murine BM SP cells, as well as analytic measures, such as single cell transplantation, that can be used to assess the functionality of SP-derived stem cells.
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Flow Cytometry-Based Cell Cycle Measurement of Mouse Hematopoietic Stem and Progenitor Cellsof cells in G. and G. in hematopoietic stem and progenitor cells. The second details the staining of bromodeoxyuridine incorporated into replicating DNA as a measure for the cycling cell fraction within a specific hematopoietic cell subset.
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1064-3745 to measure HSC function in vivo.Details retroviral and lentiThe hematopoietic stem cell (HSC) field has rapidly grown in the past several years as new technologies have been developed and the older tried and true methods have been used in new ways. Major advances in the isolation of HSC and progenit
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