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Titlebook: Helicobacter Pylori; Sinead M Smith Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 gastric cancer.in vitro i

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The Urea Breath Test for the Noninvasive Detection of ,ed urea solution ingested by the patient into carbon dioxide (CO.) and ammonia. This chapter summarizes the current use of the UBT and the utility of the “UBT and Treat” strategy compared to other strategies for the management of . infection. Different UBT methods are described as well as factors af
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Real-Time Diagnosis of , During Endoscopy by Gastric Juice Analysis,dric/achlorhydric conditions through the measurement of ammonium concentration and gastric pH. The high accuracy and feasibility of this technology enables a more accurate diagnosis and a reduced number of histologies, focusing the attention of the endoscopist on patients with high risk for cancer p
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Molecular Detection of Antibiotic-Resistant ,tic resistance rates form the basis of local guidelines for . treatment and AST may also be used as a personalized medicine approach to tailor therapy. This chapter provides an update on global antibiotic resistance rates and describes molecular mechanisms that confer . antibiotic resistance. An ove
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Endoscopic Detection of , by the Rapid Urease Test, prepyloric region, but additional biopsy specimens obtained proximally increase the sensitivity of invasive tests and are recommended, especially if the patient has recently been treated with a proton-pump inhibitor. The effects of an increased risk of sampling error and the lower prevalence of . i
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Culture-Based Antimicrobial Susceptibility Testing for ,imicrobial Susceptibility Testing (EUCAST) recommendations for fastidious microaerophilic bacteria, with some possible variation especially for the medium to be used. It is recommended to test six antibiotics by diffusion using strips charged with an antibiotic gradient in order to determine the min
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Gene Expression Analysis by Reverse Transcription Quantitative PCR,ation of specific RNA transcripts. RT-qPCR has increasingly been adopted for the detection and quantification of . across a range of sample types and applications. In addition, it is widely used to monitor host gene expression in cells and tissues in response to . infection. Outlined here is a two-s
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A Method to Investigate the ,-Associated DNA Methylome,l, and the other is the bioinformatics analysis of the methylome data. DNA methylation measurement is conducted using the commercially available array-based “Infinium Human Methylation 450K BeadChip” kit (or its updated version, Infinium MethylationEPICBeadChip). This BeadChip allows the high-throug
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Virulence Factor Genotyping,, and environmental factors influence the progression of disease from superficial gastritis to cancer. . is genetically diverse, and expression of its specific virulence factors has been linked to increased risk of more severe pathologies. Described in this chapter is a protocol for detecting import
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