| 书目名称 | Hedgehog Signaling Protocols |
| 编辑 | Natalia A. Riobo |
| 视频video | |
| 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | .The second edition of this volume provides novel protocols for the study of newly discovered function and modifications of components of the Hh pathways. The first few chapters discuss methods for stable delivery of soluble Hh proteins in cell cultures that can be adapted for in vivo studies, and mathematical models for studying Hh proteins gradient formation in tissues. The next few chapters explore studies in cell culture using fibroblasts that respond readily to Hh proteins. The book goes on to present protocols for evaluation of transcriptional activity of the Gli transcription factors in cell cultures and determine the expression level of endogenous Gli proteins. The final chapters talk about metabolic effects of noncanonical Hh signaling. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Comprehensive and authoritative, .Hedgehog Signaling Protocols, Second Edition. presents researchers with a comprehensive understanding of the Hh |
| 出版日期 | Book 2015Latest edition |
| 关键词 | Cell Culture; Cytonemes; Drosophila; Fibroblasts; G Protein-Coupled Receptor; Gli Proteins; Hedgehog (Hh) |
| 版次 | 2 |
| doi | https://doi.org/10.1007/978-1-4939-2772-2 |
| isbn_softcover | 978-1-4939-4922-9 |
| isbn_ebook | 978-1-4939-2772-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Springer Science+Business Media New York 2015 |
发表于 2025-3-21 16:28:03
