Titlebook: Hedgehog Signaling Protocols; Natalia A. Riobo Book 2015Latest edition Springer Science+Business Media New York 2015 Cell Culture.Cytoneme

Pedagogy

Controlled Delivery of Sonic Hedgehog with a Heparin-Based Coacervate,efficiency, protects its bioactivity, and provides sustained and localized release at the site of application. Shh coacervate may be injected directly or coated onto a polymeric scaffold for tissue engineering approaches, as described here.

治愈

Luciferase Reporter Assays to Study Transcriptional Activity of Hedgehog Signaling in Normal and Caf target genes. Here, we describe a protocol to measure the transcriptional activity of the HH pathway in normal and cancer cells. This technique allows studying the activity of GLI transcription factors and their modulation by drugs and/or other factors.

寻找

frozen-shoulder

Methods for Detection of Ptc1-Driven LacZ Expression in Adult Mouse Skin,be difficult, with various staining procedures yielding differential results. Thus, detailed information on staining protocols is essential for determining the ideal method for a given study. Furthermore, immunohistochemical staining of X-Gal stained tissues can provide further insight into other key players in Hh signaling activation.

Digitalis

extract

Minatory

,Investigation of Protein–Protein Interactions and Conformational Changes in Hedgehog Signaling Pathansduction, taking the example of the Hh signaling pathway, which plays a critical role in embryonic development and tissue homeostasis. A number of general guidelines as well as some key notes have been summarized as a protocol for reader’s reference.

共栖

物质

杂色

Insights into Gli Factors Ubiquitylation Methods,cribed multiple procedures to determine whether a Gli protein is ubiquitylated both in a cellular context and in vitro, in basal conditions or by different E3 ubiquitin ligases and whether these processes are associated to Gli proteasome degradation.