Titlebook: Haplotyping; Methods and Protocol Brock A. Peters,Radoje Drmanac Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive

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Gamete Binning to Achieve Haplotype-Resolved Genome Assembly,ype-specific chromosomes can be assembled independently using standard assembly tools. First applications of gamete binning revealed a haplotyping accuracy over 99%, which outperformed sequence-only or Hi-C-based haplotype-resolved genome assemblies..Availability: ..

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Sumit Sinha,Cheng-Zhong Zhangn war, such as humanitarian missions, the military medical system can expect the whole range of human diseases and conditions. Providing adequate care in the context of a military organization can be a challenge. However, careful attention to history and lessons learned can improve planning and ease the burden of meeting the mission.

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Targeted Locus Amplification and Haplotyping,ete bioinformatics pipeline for the allelic phasing of TLA data. Additionally, to illustrate the protocol, we show the ability of TLA to re-sequence and haplotype the complete cystic fibrosis transmembrane (CFTR) gene (> 200 kb in size) from patient-derived intestinal organoids.

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Low-Cost Genome-Scale Phasing with Barcode-Linked Sequencing,o sequencing costs. Whole haplotype-resolved genomes with megabase-scale phase blocks can be obtained with this method; offering researchers a unique opportunity to tackle the hurdles of de novo sequencing without being limited by a lack of resources.

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HapCUT2: A Method for Phasing Genomes Using Experimental Sequence Data,using sequence data generated using different sequencing technologies and experimental methods. In this article, we give an overview of the algorithm used by Hapcut2 and describe how to use Hapcut2 for haplotype phasing of individual genomes using different types of sequence data.

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Book 2023y materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.. ..Authoritative and cutting-edge, .Haplotyping: Methods and Protocols .aims to provide compréhensive and accessible methods to undergraduate, graduate, and established scientists..