Titlebook: Handbook of Smart Textiles; Xiaoming Tao Living reference work 20200th edition

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Wei Yuan,Chaojie Wu,Ning Zhou,Ke-Qin Zhangtection of single-copy genes in Southern blots and low abundance mRNAs in Northern blots. Labelling of (c)DNA probes involves replacement of dTTP by either digoxigenin-dUTP or biotinylated dUTP. Here we describe experimental protocols for Northern blot analysis and for genomic Southern blot using a

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Marzieh Parhizkar,Yan Zhao,Tong Lin the physical chemistry of DNA hybridization, at the end of the chapter we present a list of relevant references. In spite of a vast amount of literature on the subject the complex process of hybridization on solid support is still poorly understood. For this reasons many conditions used in this pro

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Toshihiro Hirai,Hong Xiaer on hybridization, the stringency of the buffer can easily be increased by lowering the molarity of the monovalent cations and/or increasing the temperature. For all the genomic sequencing experiments carried out in our laboratory, the washing buffers described in the ‘Step-by-Step Procedure’ were

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Mutsumi Kimurang gel to the nylon membrane. Many different parameters have to be taken into consideration: electrical resistance, strength of the electric field, diffusion, Joule heating, stability of the buffer, etc. Most of the transfer systems described in the literature so far (Towbin et al., 1979; Gibson, 19

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Tae Hwan Lim,Seong Hun Kim,Kyung Wha Ohgosaccharides with ANTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 350 18. 4 Screening of Carbohydrate Ligands for Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . 352 18. 5 Measurement of Binding Constant for the Interaction Between Protein and ANTS-Labelled Car

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Lieva Van Langenhovecribed in this book. No trouble-shooting guide can ever replace solid judgement and a thorough understanding of the biological material at hand. Therefore, it would be unrealistic to hope to present an exhaustive list of all possible problems, causes and solutions. Nevertheless, I present here the m

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