Titlebook: Handbook of Electroporation; Damijan Miklavčič Reference work 2017 Springer International Publishing AG 2017 nanosecond pulsed electropora

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Lipid Electropore Stabilizationmolecule rearrangements that are behind this phenomenon, and the visualization of the pore at the scale of size and time, in the order of nanometers and nanoseconds, respectively. Finally, the chapter also describes other approaches where pores remain open or the permeabilized state remains stable f

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Molecular Transmembrane Transport with Giant Unilamellar Vesicles (GUVs)ences on membrane structure and function. With suitable electric field parameters, it is possible to electropermeabilize the membrane leading to exchange of molecules between the inside and the outside of the vesicle. If the electropermeabilization is strong enough, membrane vesicles, tubules, and m

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Electropermeabilization and Electrostimulation by Picosecond Pulseser can be permeabilized by single, 500 ps pulse with the electric field of 190 kV/cm. This is evidenced by a lack of Ca. response in Chinese hamster ovary (CHO) cells and significant calcium uptake in murine pituitary tumor (GH3) cells and neuroblastoma-glioma hybrid (NG108) cells, which both have r

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Measurement of Molecular Transport After Electropermeabilizationon of the basic theoretical equations governing molecular transport is followed by discussions of electrical exposure systems, measurement methods, calibration of fluorescence measurements to absolute quantities, and some examples. The chapter provides perspective on the wide range of experimental d

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Electrical Conductance of Lipid Poresions to the size and numbers of pores, relying on simplified analytical formulas, is measuring the change in electrical conductance after the electropulsation..The investigation of EP with atomic detail has been possible, thanks to molecular dynamics (MD) simulations. In the past 10 years, extensive