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Titlebook: Handbook of ELISPOT ; Methods and Protocol Alexander E. Kalyuzhny Book 2024Latest edition The Editor(s) (if applicable) and The Author(s),

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Important Considerations for ELISpot Validation,LISpot protocol has been left to diverse opinions about how to interpret and tackle typical validation parameters. This chapter addresses important considerations for ELISpot validation, including the interpretations of validation parameters for a meaningful description of assay performance.
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Handbook of ELISPOT 978-1-0716-3690-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Alexander E. KalyuzhnyIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
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https://doi.org/10.1007/978-4-431-56856-8eting cells. In addition, this method can measure biological activity and immunological secretions from immune cells. In general, membrane-bound antigen allows binding of antibody secreted by B cells, or a membrane-bound analyte-specific antibody binds to the specific analyte (e.g., cytokines) elici
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Zhiming Cheng,Mark Wang,Junhua ChenISPOT), which depend on antigen-specific stimulation, increasingly use peptides to represent the antigen of interest. Besides single peptides, mixtures of peptides (peptide pools) are very frequently applied. Such peptide pools may, for example, represent entire proteins (with overlapping peptides c
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