书目名称 | Handbook of ELISPOT | 副标题 | Methods and Protocol | 编辑 | Alexander E. Kalyuzhny | 视频video | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .This fourth edition volume expands on the previous editions with discussions on the latest protocols that can be applied in different laboratory landscapes ranging from purely research to clinical diagnostic environments. This volume particularly focuses on ELISpot validation and evaluation of antibodies used in this assay. The chapters in this volume cover topics such as peptides in T-Cell assays and ELISpot applications for monitoring infectious diseases like tuberculosis and SARS-CoV2; FluoroSpot; the use of AI for studying ELISpot; and comparing ELISpot with flow cytometry, feline coronavirus (FCoV2), and memory b-cells. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and comprehensive, .Handbook of ELISPOT: Methods and Protocols, Fourth Edition. is a valuable resource to aid in the advancement of science and technology, and inspire scientists and researchers to determine and pursue new discoveries in this developing | 出版日期 | Book 2024Latest edition | 关键词 | T Cell Cytokine; Fluorospot; SARS-CoV-2; ELLA; immunocytochemistry | 版次 | 4 | doi | https://doi.org/10.1007/978-1-0716-3690-9 | isbn_softcover | 978-1-0716-3692-3 | isbn_ebook | 978-1-0716-3690-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |
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