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Titlebook: Handbook of ELISPOT ; Methods and Protocol Alexander E. Kalyuzhny Book 2024Latest edition The Editor(s) (if applicable) and The Author(s),

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发表于 2025-3-21 16:05:18 | 显示全部楼层 |阅读模式
书目名称Handbook of ELISPOT
副标题Methods and Protocol
编辑Alexander E. Kalyuzhny
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Handbook of ELISPOT ; Methods and Protocol Alexander E. Kalyuzhny Book 2024Latest edition The Editor(s) (if applicable) and The Author(s),
描述.This fourth edition volume expands on the previous editions with discussions on the latest protocols that can be applied in different laboratory landscapes ranging from purely research to clinical diagnostic environments. This volume particularly focuses on ELISpot validation and evaluation of antibodies used in this assay. The chapters in this volume cover topics such as peptides in T-Cell assays and ELISpot applications for monitoring infectious diseases like tuberculosis and SARS-CoV2; FluoroSpot; the use of AI for studying ELISpot; and comparing ELISpot with flow cytometry, feline coronavirus (FCoV2), and memory b-cells. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and comprehensive, .Handbook of ELISPOT: Methods and Protocols, Fourth Edition. is a valuable resource to aid in the advancement of science and technology, and inspire scientists and researchers to determine and pursue new discoveries in this developing
出版日期Book 2024Latest edition
关键词T Cell Cytokine; Fluorospot; SARS-CoV-2; ELLA; immunocytochemistry
版次4
doihttps://doi.org/10.1007/978-1-0716-3690-9
isbn_softcover978-1-0716-3692-3
isbn_ebook978-1-0716-3690-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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发表于 2025-3-21 23:12:06 | 显示全部楼层
Joost Hauer,Harry Timmermans,Neil Wrigleyas been proposed that the detection and measurement of memory B cells capable of secreting antibodies against donor HLA antigens could be carried out using B-cell ImmunoSpot, to better inform the degree of immune sensitization of transplant patients prior to as well as after transplantation. Such an assay is described here.
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Using PBMCs in a Multiplex FluoroSpot Assay for Detection of Innate Immune Response-Modulating Impuse of a triple-color IL-1β/IL-6/TNF-α FluoroSpot assay as a sensitive tool for quantification of the frequencies of IIRMI-activated monocytes as well as determination of the relative amount of pyrogenic cytokine(s) produced by each activated cell.
发表于 2025-3-22 11:19:38 | 显示全部楼层
1064-3745 ation advice from the experts.This fourth edition volume expands on the previous editions with discussions on the latest protocols that can be applied in different laboratory landscapes ranging from purely research to clinical diagnostic environments. This volume particularly focuses on ELISpot vali
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https://doi.org/10.1007/978-981-13-1168-0se of a triple-color IL-1β/IL-6/TNF-α FluoroSpot assay as a sensitive tool for quantification of the frequencies of IIRMI-activated monocytes as well as determination of the relative amount of pyrogenic cytokine(s) produced by each activated cell.
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Urban Biodiversity and Built EnvironmentLISpot protocol has been left to diverse opinions about how to interpret and tackle typical validation parameters. This chapter addresses important considerations for ELISpot validation, including the interpretations of validation parameters for a meaningful description of assay performance.
发表于 2025-3-23 06:52:05 | 显示全部楼层
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