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Titlebook: Handbook of Capillary Electrophoresis Applications; Hideharu Shintani (Section Director),J. Polonský ( Book 1997 Chapman & Hall 1997 bioch

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楼主: Systole
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Ayesha Kashif,Stephane Ploix,Julie Dugdale conventional on-line UV detection. Several studies have been carried out to enhance the detection sensitivity, e.g. sample preconcentration, choice of detection system including strong light source, a detector with high sensitivity and efficient optical alignment, and derivatization of analytes [1].
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Remco Hoogma,Matthias Weber,Boelie Elzen inexact collection and, in the cases where the collection vials contain a small volume of buffer, gives dilution of the fraction. The first problem is solved by calculating the migration time for fraction collection corresponding to the related migration behavior [1].
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Quantitative analysis, precision and reproducibilitytion constants in affinity CE, the molecular weight in molecular sieving mode, isoelectric point (pI) in capillary isoelectric focusing (CIEF), etc. These two pieces of information, the peak area/height and the migration time, are described in this chapter.
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Column technologyadvancement. In this chapter, recent developments in column technology are described including how to control EOF, the method of coating the surface of inner wall of the capillary, gel-filled columns, connection of capillaries and design of capillary shape.
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Detection conventional on-line UV detection. Several studies have been carried out to enhance the detection sensitivity, e.g. sample preconcentration, choice of detection system including strong light source, a detector with high sensitivity and efficient optical alignment, and derivatization of analytes [1].
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Analysis of erythropoietin by capillary electrophoresishe first functional recombinant human erythropoietin was cloned, expressed [6] and made available as one of the most celebrated therapeutic drugs in recent years. Recombinant EPO (r-EPO) and natural EPO purified from the urine showed no significant difference in their functional activity . or their carbohydrate structures [7, 8].
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https://doi.org/10.1007/978-94-017-3558-2m dodecyl sulfate)—polyacrylamide gel electrophoresis (SDS-PAGE) or isoelectric focusing (IEF) can also be carried out by CE as capillary gel electrophoresis (CGE) or capillary isoelectric focusing (CIEF).
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IPR Management to Business Management,ly high such that on a molar basis, they can catalyze the transformation of 10. to 10. moles of the substrate per minute. Enzymes provide some of the best chemical amplification processes in nature which have been used in many practical applications, including enzyme assay, enzyme-linked immunoassay (ELISA) and DNA probe assays.
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