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Titlebook: Haemophilus influenzae Protocols; Mark A. Herbert,Derek W. Hood,E. Richard Moxon Book 20031st edition Springer Science+Business Media New

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Transformation of ,,nd is rarely used for strain construction. Conjugative plasmids are common in H. ., as in other bacteria (.-.). The F plasmid of . can conjugate into H. . cells (.) (and into many other cell types), and can then transfer by conjugation from one H. . strain to another. As in ., efficient transfer of
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Diagnosis of Infection, infection at a variety of anatomical sites, especially the upper and the lower airway. For this reason, adequate diagnosis of . infections remains important. Classical microbiological diagnosis relies on growth on blood-based agar media, including chocolate agar or brain heart infusion (BHI) agar s
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Characterization of Plasmids, (.–.). Early work was hampered by the failure to easily detect extrachromosomal plasmid in clinical isolates. However, closed circular plasmid was readily detected in exconjugants following conjugal transfer of plasmid (.). Early, it was recognized that small, approx 5-kb, nonconjugative plasmid wa
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Structural Profiling of Short-Chain Lipopolysaccharides from ,,ects and serum antibody production (.). LPS have been found to exhibit a common molecular architecture consisting of at least two distinct regions: a carbohydrate containing region and a lipid moiety referred to as lipid A (.). In enteric bacteria (e.g., . spp.), the carbohydrate containing region c
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Mutagenesis of , ,he whole genome sequence and bioinformatics, there is a need for clear descriptions of the available mutagenesis tools for individual pathogens. Here we describe those that have been applied to . and discuss their advantages and limitations.
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