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Titlebook: Haemophilus influenzae Protocols; Mark A. Herbert,Derek W. Hood,E. Richard Moxon Book 20031st edition Springer Science+Business Media New

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书目名称Haemophilus influenzae Protocols
编辑Mark A. Herbert,Derek W. Hood,E. Richard Moxon
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Medicine
图书封面Titlebook: Haemophilus influenzae Protocols;  Mark A. Herbert,Derek W. Hood,E. Richard Moxon Book 20031st edition Springer Science+Business Media New
描述As the second leading bacterial cause of community-acquired pneumonia, H. influenzae accounts for many thousands of deaths per year worldwide, and is a prime candidate for the identification of targets that can lead to the development of novel vaccines and antibiotics. In Haemophilus influenzae Protocols, leading research scientists and infectious disease specialists detail in a readily reproducible format the major molecular and immunological techniques for exploring the pathogenicity of this significant bacterium. Described with step-by-step instructions to ensure robust and successful experimental results, the techniques cover plasmid analysis, proteomics, genomics, DNA array technology, gene expression, mutagenesis (transposon and nontransposon), and structural analysis. The antibody techniques presented include IVIAT, ELISA, and opsonophagocytosis assays. Also included are reviews of the pathogenesis of NTHi and Hib, methods for working with the organism, culture and storage conditions, molecular diagnosis, and systems for exploring pathogenesis in both in vitro and animal models..Comprehensive and highly practical, Haemophilus influenzae Protocols offers a unique collection o
出版日期Book 20031st edition
关键词antibiotics; bacteria; infection; infections; infectious; infectious disease; tissue; infectious diseases
版次1
doihttps://doi.org/10.1385/1592593216
isbn_softcover978-1-61737-255-1
isbn_ebook978-1-59259-321-7Series ISSN 1543-1894 Series E-ISSN 1940-6037
issn_series 1543-1894
copyrightSpringer Science+Business Media New York 2003
The information of publication is updating

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Gene Expression Technology, is the most frequently used reporter gene. In the last decade, the use of genes encoding fluorescent proteins, such as the Aequorea green fluorescent protein (GFP), has become widespread. In ., gene expression has been monitored successfully with both . and . genes.
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Opsonophagocytosis Assay Using Flow-Cytometry,ative burst within the cell. This assay using human donor peripheral blood polymorphonuclear cells (PMNs) is rapid, reproducible, and specific. Other OP assays described use HL-60 cell lines that are technically more difficult to use and require tissue culture facilities (.).
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1543-1894 sands of deaths per year worldwide, and is a prime candidate for the identification of targets that can lead to the development of novel vaccines and antibiotics. In Haemophilus influenzae Protocols, leading research scientists and infectious disease specialists detail in a readily reproducible form
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Érico Esteves Duarte,Luís Rodrigo Machado is the most frequently used reporter gene. In the last decade, the use of genes encoding fluorescent proteins, such as the Aequorea green fluorescent protein (GFP), has become widespread. In ., gene expression has been monitored successfully with both . and . genes.
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