书目名称 | Haemophilus influenzae Protocols | 编辑 | Mark A. Herbert,Derek W. Hood,E. Richard Moxon | 视频video | | 概述 | Includes supplementary material: | 丛书名称 | Methods in Molecular Medicine | 图书封面 |  | 描述 | As the second leading bacterial cause of community-acquired pneumonia, H. influenzae accounts for many thousands of deaths per year worldwide, and is a prime candidate for the identification of targets that can lead to the development of novel vaccines and antibiotics. In Haemophilus influenzae Protocols, leading research scientists and infectious disease specialists detail in a readily reproducible format the major molecular and immunological techniques for exploring the pathogenicity of this significant bacterium. Described with step-by-step instructions to ensure robust and successful experimental results, the techniques cover plasmid analysis, proteomics, genomics, DNA array technology, gene expression, mutagenesis (transposon and nontransposon), and structural analysis. The antibody techniques presented include IVIAT, ELISA, and opsonophagocytosis assays. Also included are reviews of the pathogenesis of NTHi and Hib, methods for working with the organism, culture and storage conditions, molecular diagnosis, and systems for exploring pathogenesis in both in vitro and animal models..Comprehensive and highly practical, Haemophilus influenzae Protocols offers a unique collection o | 出版日期 | Book 20031st edition | 关键词 | antibiotics; bacteria; infection; infections; infectious; infectious disease; tissue; infectious diseases | 版次 | 1 | doi | https://doi.org/10.1385/1592593216 | isbn_softcover | 978-1-61737-255-1 | isbn_ebook | 978-1-59259-321-7Series ISSN 1543-1894 Series E-ISSN 1940-6037 | issn_series | 1543-1894 | copyright | Springer Science+Business Media New York 2003 |
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